RT info:eu-repo/semantics/article
T1 Oxidative stress mediates epigenetic modifications and the expression of miRNAs and genes related to apoptosis in diabetic retinopathy patients
A1 Karam Palos, Sarah
A1 Andrés Blasco, Irene
A1 Campos Borges, Cristina
A1 Zanón Moreno, Vicente
A1 Gallego Martínez, Alex
A1 Alegre Ituarte, Victor
A1 García Medina, Jose J.
A1 Pastor Idoate, Salvador
A1 Sellés Navarro, Inmaculada
A1 Vila Arteaga, Jorge
A1 Lleó Perez, Antonio V.
A1 Pinazo Durán, Maria Dolores
K1 Diabetic retinopathy
K1 Diabetes
K1 Ophthalmology
K1 Retinopatía diabética
K1 Eye - Diseases
K1 Ojo - Enfermedades y defectos
K1 Oxidative stress
K1 Estrés oxidativo
K1 MicroRNAs
K1 Genes
K1 Apoptosis
K1 Epigenetics
K1 Genetics
K1 Genetica
K1 Nervous system - Degeneration
K1 Sistema nervioso - Degeneración
K1 3201.09 Oftalmología
K1 3205.07 Neurología
AB Knowledge on the underlying mechanisms and molecular targets for managing the ocular complications of type 2 diabetes mellitus (T2DM) remains incomplete. Diabetic retinopathy (DR) is a major cause of irreversible visual disability worldwide. By using ophthalmological and molecular-genetic approaches, we gathered specific information to build a data network for deciphering the crosslink of oxidative stress (OS) and apoptosis (AP) processes, as well as to identify potential epigenetic modifications related to noncoding RNAs in the eyes of patients with T2DM. A total of 120 participants were recruited, being classified into two groups: individuals with T2MD (T2MDG, n = 67), divided into a group of individuals with (+DR, n = 49) and without (−DR, n = 18) DR, and a control group (CG, n = 53). Analyses of compiled data reflected significantly higher plasma levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and significantly lower total antioxidant capacity (TAC) in the +DR patients compared with the −DR and the CG groups. Furthermore, the plasma caspase-3 (CAS3), highly involved in apoptosis (AP), showed significantly higher values in the +DR group than in the −DR patients. The microRNAs (miR) hsa-miR 10a-5p and hsa-miR 15b-5p, as well as the genes BCL2L2 and TP53 involved in these pathways, were identified in relation to DR clinical changes. Our data suggest an interaction between OS and the above players in DR pathogenesis. Furthermore, potential miRNA-regulated target genes were identified in relation to DR. In this concern, we may raise new diagnostic and therapeutic challenges that hold the potential to significantly improve managing the diabetic eye.
PB MDPI
SN 2077-0383
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/67213
UL https://uvadoc.uva.es/handle/10324/67213
LA eng
NO Journal of Clinical Medicine, 2024, Vol. 13, Nº. 1, 74
NO Producción Científica
DS UVaDOC
RD 16-ago-2024