RT info:eu-repo/semantics/article
T1 Phytoconstituents and ergosterol biosynthesis-targeting antimicrobial activity of nutmeg (Myristica fragans Houtt.) against phytopathogens
A1 Cruz, Adriana
A1 Sánchez Hernández, Eva
A1 Teixeira, Ana
A1 Oliveira, Rui
A1 Cunha, Ana
A1 Martín Ramos, Pablo
K1 Biopesticide
K1 phenylpropenes
K1 Indole alkaloids
K1 myristica fragans
K1 Plant extracts
K1 Fusarium culmorum
K1 Microbiology
K1 Plant science
K1 2302.01 Alcaloides
K1 3102 Ingeniería Agrícola
K1 3106 Ciencia Forestal
K1 2417.09 Fitopatología
K1 2414 Microbiología
K1 3101.05 Fungicidas
AB In recent years, nutmeg (Myristica fragans Houtt.) has attracted considerable attention in the field of phytochemistry due to its diverse array of bioactive compounds. However, the potential application of nutmeg as a biorational for crop protection has been insufficiently explored. This study investigated the constituents of a nutmeg hydroethanolic extract via gas chromatography-mass spectrometry and vibrational spectroscopy. The research explored the extract’s activity against phytopathogenic fungi and oomycetes, elucidating its mechanism of action. The phytochemical profile revealed fatty acids (including tetradecanoic acid, 9-octadecenoic acid, n-hexadecanoic acid, dodecanoic acid, and octadecanoic acid), methoxyeugenol, and elemicin as the main constituents. Previously unreported phytochemicals included veratone, gelsevirine, and montanine. Significant radial growth inhibition of mycelia was observed against Botrytis cinerea, Colletotrichum acutatum, Diplodia corticola, Phytophthora cinnamomi, and especially against Fusarium culmorum. Mode of action investigation, involving Saccharomyces cerevisiae labeled positively with propidium iodide, and a mutant strain affected in ERG6, encoding sterol C-24 methyltransferase, suggested that the extract induces a necrotic type of death and targets ergosterol biosynthesis. The evidence presented underscores the potential of nutmeg as a source of new antimicrobial agents, showing particular promise against F. culmorum.
PB MDPI
SN 1420-3049
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/67297
UL https://uvadoc.uva.es/handle/10324/67297
LA eng
NO Molecules, 2024, Vol. 29, Nº. 2, 471
NO Producción Científica
DS UVaDOC
RD 25-dic-2024