RT info:eu-repo/semantics/article
T1 Edodin: A new type of toxin from shiitake mushroom (Lentinula edodes) that inactivates mammalian ribosomes
A1 Citores González, Lucía
A1 Ragucci, Sara
A1 Gay, Claudia C.
A1 Russo, Rosita
A1 Chambery, Angela
A1 Di Maro, Antimo
A1 Iglesias Álvarez, María del Rosario
A1 Ferreras Rodríguez, José Miguel
K1 Protein Synthesis
K1 Pyridoxal phosphate
K1 Ribosome inactivating protein
K1 Ribotoxin
K1 Lentinula edodes
K1 Shiitake
K1 rRNA N-glycosylase
K1 Toxin
K1 2302.27 Proteínas
K1 2301 Química Analítica
K1 2302 Bioquímica
K1 3214 Toxicología
AB Ribosome-inactivating proteins (RIPs) are a group of proteins with rRNA N-glycosylase activity that irreversibly inhibit protein synthesis and consequently cause cell death. Recently, an RIP called ledodin has been found in shiitake; it is cytotoxic, strongly inhibits protein synthesis, and shows rRNA N-glycosylase activity. In this work, we isolated and characterized a 50 kDa cytotoxic protein from shiitake that we named edodin. Edodin inhibits protein synthesis in a mammalian cell-free system, but not in insect-, yeast-, and bacteria-derived systems. It exhibits rRNA N-glycosylase and DNA-nicking activities, which relate it to plant RIPs. It was also shown to be toxic to HeLa and COLO 320 cells. Its structure is not related to other RIPs found in plants, bacteria, or fungi, but, instead, it presents the characteristic structure of the fold type I of pyridoxal phosphate-dependent enzymes. Homologous sequences have been found in other fungi of the class Agaricomycetes; thus, edodin could be a new type of toxin present in many fungi, some of them edible, which makes them of great interest in health, both for their involvement in food safety and for their potential biomedical and biotechnological applications.
PB MDPI
SN 2072-6651
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/67417
UL https://uvadoc.uva.es/handle/10324/67417
LA eng
NO Toxins, 2024, Vol. 16, Nº. 4, 185
NO Producción Científica
DS UVaDOC
RD 27-dic-2024