RT info:eu-repo/semantics/article
T1 Predominantly pro-inflammatory phenotype with mixed M1/M2 polarization of peripheral blood classical monocytes and monocyte-derived macrophages among patients with excessive ethanol intake
A1 Fernández Regueras, María
A1 Carbonell, Cristina
A1 Salete Granado, Daniel
A1 García, Juan Luis
A1 Gragera, Marcos
A1 Pérez Nieto, María Ángeles
A1 Morán Plata, Francisco Javier
A1 Mayado, Andrea
A1 Torres, Jorge Luis
A1 Corchete Sánchez, Luis Antonio
A1 Usategui Martín, Ricardo
A1 Bueno Martínez, Elena
A1 Rojas Pirela, Maura
A1 Sabio, Guadalupe
A1 González Sarmiento, Rogelio
A1 Orfao, Alberto
A1 Laso, Francisco Javier
A1 Almeida, Julia
A1 Marcos, Miguel
K1 Alcohol Use Disorders
K1 Excessive alcohol drinkers
K1 Monocyte/macrophage phenotype
K1 M1
K1 M2
K1 Innate immunity
K1 Flow cytometry
K1 MicroRNA
K1 6113.01 Alcoholismo
K1 2412 Inmunología
K1 2415 Biología Molecular
K1 2407 Biología Celular
AB Excessive alcohol consumption impairs the immune system, induces oxidative stress, and triggers the activation of peripheral blood (PB) monocytes, thereby contributing to alcoholic liver disease (ALD). We analyzed the M1/M2 phenotypes of circulating classical monocytes and macrophage-derived monocytes (MDMs) in excessive alcohol drinkers (EADs). PB samples from 20 EADs and 22 healthy controls were collected for isolation of CD14+ monocytes and short-term culture with LPS/IFNγ, IL4/IL13, or without stimulation. These conditions were also used to polarize MDMs into M1, M2, or M0 phenotypes. Cytokine production was assessed in the blood and culture supernatants. M1/M2-related markers were analyzed using mRNA expression and surface marker detection. Additionally, the miRNA profile of CD14+ monocytes was analyzed. PB samples from EADs exhibited increased levels of pro-inflammatory cytokines. Following short-term culture, unstimulated blood samples from EADs showed higher levels of soluble TNF-α and IL-8, whereas monocytes expressed increased levels of surface TNF-α and elevated mRNA expression of pro-inflammatory cytokines and inducible nitric oxide synthase. MDMs from EADs showed higher levels of TNF-α and CD206 surface markers and increased IL-10 production. LPS/IFNγ induced higher mRNA expression of Nrf2 only in the controls. miRNA analysis revealed a distinctive miRNA profile that is potentially associated with liver carcinogenesis and ALD through inflammation and oxidative stress. This study confirms the predominantly pro-inflammatory profile of PB monocytes among EADs and suggests immune exhaustion features in MDMs.
PB MDPI
SN 2076-3921
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/67727
UL https://uvadoc.uva.es/handle/10324/67727
LA eng
NO Antioxidants, 2023, Vol. 12, Nº. 9, 1708
NO Producción Científica
DS UVaDOC
RD 24-nov-2024