RT info:eu-repo/semantics/article T1 Chronic intermittent hypoxia-induced dysmetabolism is associated with hepatic oxidative stress, mitochondrial dysfunction and inflammation A1 Fernandes, Joana L. A1 Martins, Fátima O. A1 Olea Fraile, Elena A1 Prieto Lloret, Jesús A1 Braga, Patrícia C. A1 Sacramento, Joana F. A1 Sequeira, Catarina O. A1 Negrinho, Ana P. A1 Pereira, Sofia A. A1 Alves, Marco G. A1 Rocher Martín, María Asunción A1 Conde, Silvia V. K1 Sleep disorders K1 Obstructive sleep apnean K1 Chronic intermittent hypoxia K1 Insulin resistance K1 Metabolic disorders K1 Mitochondrial dysfunction K1 Inflammation K1 32 Ciencias Médicas K1 2411.08 Metabolismo Humano K1 2415 Biología Molecular K1 2302 Bioquímica AB The association between obstructive sleep apnea (OSA) and metabolic disorders is well-established; however, the underlying mechanisms that elucidate this relationship remain incompletely understood. Since the liver is a major organ in the maintenance of metabolic homeostasis, we hypothesize that liver dysfunction plays a crucial role in the pathogenesis of metabolic dysfunction associated with obstructive sleep apnea (OSA). Herein, we explored the underlying mechanisms of this association within the liver. Experiments were performed in male Wistar rats fed with a control or high fat (HF) diet (60% lipid-rich) for 12 weeks. Half of the groups were exposed to chronic intermittent hypoxia (CIH) (30 hypoxic (5% O2) cycles, 8 h/day) that mimics OSA, in the last 15 days. Insulin sensitivity and glucose tolerance were assessed. Liver samples were collected for evaluation of lipid deposition, insulin signaling, glucose homeostasis, hypoxia, oxidative stress, antioxidant defenses, mitochondrial biogenesis and inflammation. Both the CIH and HF diet induced dysmetabolism, a state not aggravated in animals submitted to HF plus CIH. CIH aggravates hepatic lipid deposition in obese animals. Hypoxia-inducible factors levels were altered by these stimuli. CIH decreased the levels of oxidative phosphorylation complexes in both groups and the levels of SOD-1. The HF diet reduced mitochondrial density and hepatic antioxidant capacity. The CIH and HF diet produced alterations in cysteine-related thiols and pro-inflammatory markers. The results obtained suggest that hepatic mitochondrial dysfunction and oxidative stress, leading to inflammation, may be significant factors contributing to the development of dysmetabolism associated with OSA. PB MDPI SN 2076-3921 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/67885 UL https://uvadoc.uva.es/handle/10324/67885 LA eng NO Antioxidants, 2023, Vol. 12, Nº. 11, 1910 NO Producción Científica DS UVaDOC RD 12-nov-2024