RT info:eu-repo/semantics/article T1 Modulation of the aromatic profile of verdejo wine through sequential inoculation of Wickerhamomyces anomalus and Saccharomyces cerevisiae A1 López Enríquez, Lorena A1 Vila Crespo, Josefina María A1 Rodríguez Nogales, José Manuel A1 Fernández Fernández, Encarnación A1 Ruipérez Prádanos, Violeta K1 Wine K1 Indigenous yeasts K1 Sequential inoculation K1 Vinos blancos K1 Non-Saccharomyces K1 Wine quality K1 Verdejo (Vino) K1 Aromatic profile K1 2414 Microbiología K1 3309.29 Vino K1 3309 Tecnología de Los Alimentos K1 3102 Ingeniería Agrícola AB Two strains of Saccharomyces cerevisiae (Sc01 and Sc02) and one strain of Wickerhamomyces anomalus (Wa) were isolated from organic Verdejo spontaneous fermentations and used for the development of experimental winemaking. Sc01 and Sc02 represented 52.7% of the population of the Saccharomyces strains isolated throughout the fermentation process. W. anomalus appeared as the predominant species among the non-Saccharomyces yeasts. Wa turned out to be the strain of this species with the shortest lag phase and positive enzymatic activities, and it was selected for white wine production. Fermentations with unique inoculation of S. cerevisiae strains were compared with sequential inoculation with W. anomalus. The results showed that the sequential inoculations did not affect the fermentation kinetics or physicochemical characteristics of the wines compared with the unique inoculations. However, this study identified a significant impact on the aromatic profiles of the produced wines due to the sequential inoculations. This modification resulted in a similar new aromatic profile in both sequential inoculations, demonstrating common characteristics related to the contribution of W. anomalus. In general, the sequential fermentations were mainly characterized by lower levels of acetate esters and an increase in ethyl acetate levels, whereas lower levels of ethyl octanoate and ethyl dec-9-enoate were detected. Propan-1-ol and butan-1-ol showed an increase in the sequential fermentations, while 4-methylpentan-1-ol and 2-phenylethanol were found in lower concentrations. These results highlight the great influence that the presence of specific strains of native non-Saccharomyces yeasts exerts on the characteristics of elaborate wines. PB MDPI SN 2311-5637 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/67935 UL https://uvadoc.uva.es/handle/10324/67935 LA eng NO Fermentation, 2023, Vol. 9, Nº. 11, 977 NO Producción Científica DS UVaDOC RD 22-dic-2024