RT info:eu-repo/semantics/article
T1 Smilax aspera L. Leaf and fruit extracts as antibacterial agents for crop protection
A1 Fontana, Riccardo
A1 Sánchez Hernández, Eva
A1 Martín Ramos, Pablo
A1 Martín Gil, Jesús
A1 Marconi, Peggy
K1 Antibacterial agents
K1 Antibacterial
K1 Antiinfecciosos
K1 Agentes Antibacterianos
K1 Bacterial diseases
K1 Enfermedades bacterianas
K1 Plant diseases
K1 Plant Pathology
K1 Plantas - Enfermedades y plagas
K1 Plants, Protection of
K1 Cultivos - Protección
K1 Plant science
K1 Natural products
K1 Productos naturales
K1 3102 Ingeniería Agrícola
K1 3103 Agronomía
K1 3108 Fitopatología
AB Smilax aspera L. (commonly known as sarsaparilla) is recognized for its composition rich in flavonoids, phenylpropanoids, steroidal saponins, stilbenoids, and tannins, exhibiting anti-inflammatory, cytotoxic, and antimicrobial properties. This study investigates the hydromethanolic extracts of its leaves and fruits through vibrational spectroscopy and gas chromatography–mass spectrometry, evaluating their potential as biorationals for safeguarding crops. Analysis of S. aspera leaf and fruit extracts revealed the presence of phytochemicals such as lactones and other furan derivatives. In vitro assessments against three phytopathogens—Erwinia amylovora, Pseudomonas syringae pv. actinidiae, and Xanthomonas campestris pv. campestris—demonstrated strong antibacterial activity, with minimum inhibitory concentration (MIC) values of 1500 μg·mL−1 for both extracts. Biofilm tests indicated that the leaf extract reduced biofilm formation by 78–85%, while the fruit extract led to a reduction of 73–92.5%. At a concentration of 750 µg·mL−1, the extracts caused a decrease in amylovoran synthesis by 41–58%. Additionally, noticeable alterations in membrane permeability were observed at MIC and MIC/2 doses. Subsequent in vivo trials conducted on Pyrus communis L. trees utilizing the combined aerial part extract yielded substantial protection against E. amylovora at a dose of 1500 μg·mL−1, reaching 80% wilting reduction for the leaf extract. The findings presented herein cast S. aspera extracts as a promising natural-based treatment against these bacterial phytopathogens.
PB MDPI
SN 2073-4395
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/68068
UL https://uvadoc.uva.es/handle/10324/68068
LA eng
NO Agronomy, 2024, Vol. 14, Nº. 2, 383
NO Producción Científica
DS UVaDOC
RD 03-dic-2024