RT info:eu-repo/semantics/article
T1 Effects of 2-Deoxy-D-Glucose on In Vitro Cat Carotid Body
A1 Obeso Cáceres, Ana María de la Luz
A1 Almaraz Gómez, Laura
A1 González, Constancio
K1 Neurofisiología
AB The process of chemosensory transduction in the arterial chemoreceptors is not well understood. According to the metabolic hypothesisof chemoreception, a decrease in arterial pO 2 will produce a decrease in the adenosine triphosphate (ATP) content in the chcmosensorytype I cells, leading to release of a neurotransmitter and increased sensory neural activity. There is a paucity of direct experimentalsupport for this hypothesis, and in the present work, we have tested the postulates of the metabolic hypothesis in an in vitropreparation of cat carotid body using 2-deoxy-D-glucose as an ATP-depleting agent. This preparation, when superfused with Tyrodecontaining 5 mM Na+-pyruvate instead of glucose, responds normally to hypoxia, low pH and acetylcholinc, and maintains normalATP levels. Under these conditions, 2-deoxy-D-glucose is a chemostimulant, i.e. electrical activity in the carotid sinus nerve is increased,with a threshold concentration of 0.25 mM and a maximum response at about 2-4 raM. These concentrations of 2-deoxyglucoseevoke a dose-dependent release of [3H]dopamine (synthesized from pH]tyrosine) from the carotid bodies which parallels theelectrical activity. The 2-deoxy-D-glucose-evoked release and electrical activity is dependent on the presence of extracellular Ca 2+ .These same concentrations of 2-deoxy-D-glucose significantly reduce the ATP content of the carotid bodies. The events postulated bvthe metabolic hypothesis, i.e. decrease in ATP content, release of a putative neurotransminer and activation of the sensory nerve endingsare found to occur simultaneously. A possible cause-effect relationship between these three events is discussed.
PB Elsevier
SN 00006-8993
YR 1986
FD 1986
LK http://uvadoc.uva.es/handle/10324/6825
UL http://uvadoc.uva.es/handle/10324/6825
LA eng
NO Brain Research 371(1986) 25-36
NO Producción Científica
DS UVaDOC
RD 03-dic-2024