RT info:eu-repo/semantics/article
T1 Silica-calcium-alginate hydrogels for the co-immobilization of glucose oxidase and catalase to reduce the glucose in grape must
A1 Bosque Fernández, David del
A1 Vila Crespo, Josefina María
A1 Ruipérez Prádanos, Violeta
A1 Fernández Fernández, Encarnación
A1 Rodríguez Nogales, José Manuel
K1 Encapsulation
K1 Polimers
K1 Biomaterials
K1 Biomateriales
K1 Bioengineering
K1 Organic chemistry
K1 Food - Biotechnology
K1 Grape products
K1 Uvas - Análisis
K1 Mostos - Análisis
K1 Wines and wine making
K1 Vinos y vinificación
K1 Materials science
K1 Sol-gel
K1 Materials science
K1 Food science
K1 Agriculture
K1 2206.10 Polímeros
K1 3102 Ingeniería Agrícola
K1 2306 Química Orgánica
K1 3312 Tecnología de Materiales
K1 3309 Tecnología de Los Alimentos
AB Higher temperatures due to climate change are causing greater sugar production in grapes and more alcoholic wines. The use of glucose oxidase (GOX) and catalase (CAT) in grape must is a biotechnological green strategy to produce reduced-alcohol wines. GOX and CAT were effectively co-immobilized by sol-gel entrapment in silica-calcium-alginate hydrogel capsules. The optimal co-immobilization conditions were achieved at a concentration of the colloidal silica, sodium silicate and sodium alginate of 7.38%, 0.49% and 1.51%, respectively, at pH 6.57. The formation of a porous silica-calcium-alginate structure was confirmed by environmental scanning electron microscopy and the elemental analysis of the hydrogel by X-ray spectroscopy. The immobilized GOX showed a Michaelis–Menten kinetic, while the immobilized CAT fits better to an allosteric model. Immobilization also conferred superior GOX activity at low pH and temperature. The capsules showed a good operational stability, as they could be reused for at least 8 cycles. A substantial reduction of 26.3 g/L of glucose was achieved with encapsulated enzymes, which corresponds to a decrease in potential alcoholic strength of must of about 1.5% vol. These results show that co-immobilized GOX and CAT in silica-calcium-alginate hydrogels is a promising strategy to produce reduced-alcohol wines.
PB MDPI
SN 2310-2861
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/69076
UL https://uvadoc.uva.es/handle/10324/69076
LA eng
NO Gels, 2023, Vol. 9, Nº. 4, 320
NO Producción Científica
DS UVaDOC
RD 27-jul-2024