RT info:eu-repo/semantics/article
T1 Bioglues based on an elastin-like recombinamer: effect of tannic acid as an additive on tissue adhesion and cytocompatibility
A1 Sarisoy, Alp
A1 Acosta Rodríguez, Sergio
A1 Rodríguez Cabello, José Carlos
A1 Czichowski, Phillip
A1 Kopp, Alexander
A1 Jockenhoevel, Stefan
A1 Fernández Colino, Alicia
K1 Adhesives
K1 Adhesivos
K1 Biomaterials
K1 Biomateriales
K1 Biomedical materials
K1 Polymers
K1 Proteins
K1 Medicine
K1 Polímeros recombinantes
K1 Tejidos (Histología)
K1 Bones
K1 Huesos
K1 Skin
K1 Piel
K1 Organic chemistry
K1 Inorganic chemistry
K1 Molecular biology
K1 Medical technology
K1 2206.10 Polímeros
K1 2302.27 Proteínas
K1 2306 Química Orgánica
K1 2303 Química Inorgánica
K1 32 Ciencias Médicas
K1 2415 Biología Molecular
K1 3314 Tecnología Médica
AB More than 260 million surgical procedures are performed worldwide each year. Although sutures and staples are widely used to reconnect tissues, they can cause further damage and increase the risk of infection. Bioadhesives have been proposed as an alternative to reconnect tissues. However, clinical adhesives that combine strong adhesion with cytocompatibility have yet to be developed. In this study, we explored the production of adhesives based on protein-engineered polymers bioinspired by the sequence of elastin (i.e., elastin-like recombinamers, ELRs). We hypothesized that the combination of polyphenols (i.e., tannic acid, TA) and ELRs would produce an adhesive coacervate (ELR+TA), as reported for other protein polymers such as silk fibroin (SF). Notably, the adhesion of ELR alone surpassed that of ELR+TA. Indeed, ELR alone achieved adhesive strengths of 88.8 ± 33.2 kPa and 17.0 ± 2.0 kPa on porcine bone and skin tissues, respectively. This surprising result led us to explore a multicomponent bioadhesive to encompass the complementary roles of elastin (mimicked here by ELR) and silk fibroin (SF), and subsequently mirror more closely the multicomponent nature of the extracellular matrix. Tensile testing showed that ELR+SF achieved an adhesive strength of 123.3 ± 60.2 kPa on porcine bone and excellent cytocompatibility. To express this in a more visual and intuitive way, a small surface of only 2.5 cm2 was able to lift at least 2 kg of weight. This opens the door for further studies focusing on the ability of protein-engineered polymers to adhere to biological tissues without further chemical modification for applications in tissue engineering.
PB MDPI
SN 1422-0067
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/69182
UL https://uvadoc.uva.es/handle/10324/69182
LA eng
NO International Journal of Molecular Sciences, 2023, Vol. 24, Nº. 7, 6776
NO Producción Científica
DS UVaDOC
RD 22-dic-2024