RT info:eu-repo/semantics/article T1 Bioglues based on an elastin-like recombinamer: effect of tannic acid as an additive on tissue adhesion and cytocompatibility A1 Sarisoy, Alp A1 Acosta Rodríguez, Sergio A1 Rodríguez Cabello, José Carlos A1 Czichowski, Phillip A1 Kopp, Alexander A1 Jockenhoevel, Stefan A1 Fernández Colino, Alicia K1 Adhesives K1 Adhesivos K1 Biomaterials K1 Biomateriales K1 Biomedical materials K1 Polymers K1 Proteins K1 Medicine K1 Polímeros recombinantes K1 Tejidos (Histología) K1 Bones K1 Huesos K1 Skin K1 Piel K1 Organic chemistry K1 Inorganic chemistry K1 Molecular biology K1 Medical technology K1 2206.10 Polímeros K1 2302.27 Proteínas K1 2306 Química Orgánica K1 2303 Química Inorgánica K1 32 Ciencias Médicas K1 2415 Biología Molecular K1 3314 Tecnología Médica AB More than 260 million surgical procedures are performed worldwide each year. Although sutures and staples are widely used to reconnect tissues, they can cause further damage and increase the risk of infection. Bioadhesives have been proposed as an alternative to reconnect tissues. However, clinical adhesives that combine strong adhesion with cytocompatibility have yet to be developed. In this study, we explored the production of adhesives based on protein-engineered polymers bioinspired by the sequence of elastin (i.e., elastin-like recombinamers, ELRs). We hypothesized that the combination of polyphenols (i.e., tannic acid, TA) and ELRs would produce an adhesive coacervate (ELR+TA), as reported for other protein polymers such as silk fibroin (SF). Notably, the adhesion of ELR alone surpassed that of ELR+TA. Indeed, ELR alone achieved adhesive strengths of 88.8 ± 33.2 kPa and 17.0 ± 2.0 kPa on porcine bone and skin tissues, respectively. This surprising result led us to explore a multicomponent bioadhesive to encompass the complementary roles of elastin (mimicked here by ELR) and silk fibroin (SF), and subsequently mirror more closely the multicomponent nature of the extracellular matrix. Tensile testing showed that ELR+SF achieved an adhesive strength of 123.3 ± 60.2 kPa on porcine bone and excellent cytocompatibility. To express this in a more visual and intuitive way, a small surface of only 2.5 cm2 was able to lift at least 2 kg of weight. This opens the door for further studies focusing on the ability of protein-engineered polymers to adhere to biological tissues without further chemical modification for applications in tissue engineering. PB MDPI SN 1422-0067 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/69182 UL https://uvadoc.uva.es/handle/10324/69182 LA eng NO International Journal of Molecular Sciences, 2023, Vol. 24, Nº. 7, 6776 NO Producción Científica DS UVaDOC RD 09-nov-2024