RT info:eu-repo/semantics/article
T1 Antibacterial activity of Ginkgo biloba extracts against Clavibacter michiganensis subsp. michiganensis, Pseudomonas spp., and Xanthomonas vesicatoria
A1 Sánchez Hernández, Eva
A1 González García, Vicente
A1 Palacio Bielsa, Ana
A1 Lorenzo Vidal, Belén
A1 Buzón Durán, Laura
A1 Martín Gil, Jesús
A1 Martín Ramos, Pablo
K1 Plant Pathology
K1 Plantas - Enfermedades y plagas
K1 Phytopathogenic bacteria
K1 Bacterias fitopatógenas
K1 Antibacterial activity
K1 Mass spectrometry
K1 Espectrometría de masas
K1 Food - Biotechnology
K1 Tomatoes
K1 Tomates - Cultivo
K1 Guisantes
K1 Agriculture
K1 Plant science
K1 Horticulture
K1 Food - Biotechnology
K1 3108 Fitopatología
K1 3102 Ingeniería Agrícola
K1 3107 Horticultura
K1 3309 Tecnología de Los Alimentos
AB Phytopathogenic bacteria represent a risk to global food production by impacting a variety of crops. The aim of this study was to characterize the contents of bioactive constituents in extracts from Ginkgo biloba L. leaves and fruits and test their activity against six phytopathogenic bacteria that affect horticultural crops. Gas chromatography–mass spectrometry (GC−MS) was used for the chemical profiling of the aqueous methanol extracts, and their bacteriostatic activity against Clavibacter michiganensis subsp. michiganensis, Pseudomonas cichorii, Pseudomonas syringae pv. pisi, Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. tomato, and Xanthomonas vesicatoria (formerly Xanthomonas campestris pv. vesicatoria) was determined in vitro through the agar dilution method. The protective effect of the leaf extract was tested in vivo against the most relevant bacterial pathogens based on their economic/scientific importance, i.e., C. michiganensis subsp. michiganensis and P. syringae pv. pisi, in tomato (Solanum lycopersicum L.) and pea (Pisum sativum L.) plants, respectively, under greenhouse conditions. The GC−MS characterization of G. biloba extracts revealed the presence of dihydro-4-hydroxy-2(3H)-furanone, 2,4-dimethyl-3-hexanol, catechol, 3-O-methyl-D-fructose, 4,6-di-O-methyl-α-D-galactose, methyl 2-O-methyl-α-D-xylofuranoside, and 3-methyl mannoside. In vitro growth inhibition tests showed that, while the fruit extract had no activity, the leaf extract exhibited minimum inhibitory concentrations between 500 and 1000 μg mL−1, which may be attributed to the presence of 2,4-dimethyl-3-hexanol and catechol. In vivo tests of the leaf extract demonstrated full protection in tomato and pea plants at 1000 and 1500 μg∙mL−1, respectively. The results indicate that G. biloba leaves may be employed as a biorational source for integrated pest management in horticulture.
PB MDPI
SN 2311-7524
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/69206
UL https://uvadoc.uva.es/handle/10324/69206
LA eng
NO Horticulturae, 2023, Vol. 9, Nº. 4, 461
NO Producción Científica
DS UVaDOC
RD 27-jul-2024