RT info:eu-repo/semantics/article
T1 Characterization of the antitumor potential of extracts of Cannabis sativa strains with high CBD content in human neuroblastoma
A1 Sánchez Sánchez, Laura
A1 García, Javier
A1 Fernández, Roberto
A1 Noskova, Ekaterina
A1 Egiguren Ortiz, June
A1 Gulak, Marina
A1 Ochoa, Eneko
A1 Laso, Antonio
A1 Oiarbide, Mikel
A1 Santos, José Ignacio
A1 Andrés, María Fe
A1 González Coloma, Azucena
A1 Adell, Albert
A1 Astigarraga, Egoitz
A1 Barreda Gómez, Gabriel
K1 Cannabis
K1 Cannabis - Therapeutic use
K1 Cannabis - Uso terapeútico
K1 Cannabinoids
K1 Plant extracts
K1 Drugs
K1 Drogas
K1 Medicinal plants
K1 Plantas medicinales
K1 Pharmacology
K1 Neuroblastoma
K1 Cancer
K1 Cancer - Traitement
K1 Cáncer - Tratamiento
K1 Oncology
K1 Antitumor
K1 3209 Farmacología
K1 2302.21 Biología Molecular
K1 3201.01 Oncología
AB Cannabis has been used for decades as a palliative therapy in the treatment of cancer. This is because of its beneficial effects on the pain and nausea that patients can experience as a result of chemo/radiotherapy. Tetrahydrocannabinol and cannabidiol are the main compounds present in Cannabis sativa, and both exert their actions through a receptor-mediated mechanism and through a non-receptor-mediated mechanism, which modulates the formation of reactive oxygen species. These oxidative stress conditions might trigger lipidic changes, which would compromise cell membrane stability and viability. In this sense, numerous pieces of evidence describe a potential antitumor effect of cannabinoid compounds in different types of cancer, although controversial results limit their implementation. In order to further investigate the possible mechanism involved in the antitumoral effects of cannabinoids, three extracts isolated from Cannabis sativa strains with high cannabidiol content were analyzed. Cell mortality, cytochrome c oxidase activity and the lipid composition of SH-SY5Y cells were determined in the absence and presence of specific cannabinoid ligands, with and without antioxidant pre-treatment. The cell mortality induced by the extracts in this study appeared to be related to the inhibition of the cytochrome c oxidase activity and to the THC concentration. This effect on cell viability was similar to that observed with the cannabinoid agonist WIN55,212-2. The effect was partially blocked by the selective CB1 antagonist AM281, and the antioxidant α-tocopherol. Moreover, certain membrane lipids were affected by the extracts, which demonstrated the importance of oxidative stress in the potential antitumoral effects of cannabinoids.
PB MDPI
SN 1422-0067
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/69397
UL https://uvadoc.uva.es/handle/10324/69397
LA eng
NO International Journal of Molecular Sciences, 2023, Vol. 24, Nº. 4, 3837
NO Producción Científica
DS UVaDOC
RD 10-nov-2024