RT info:eu-repo/semantics/article
T1 A coating based on bioactive compounds from Streptomyces spp. and chitosan oligomers to control Botrytis cinerea preserves the quality and Improves the shelf life of table grapes
A1 Buzón Durán, Laura
A1 Sánchez Hernández, Eva
A1 Sánchez Báscones, Mercedes
A1 García González, María Cruz
A1 Hernández Navarro, Salvador
A1 Correa Guimaraes, Adriana
A1 Martín Ramos, Pablo
K1 Botrytis cinerea
K1 Fungi
K1 Plant diseases
K1 Plantas - Enfermedades y plagas
K1 Fruit - Postharvest diseases and injuries
K1 Frutas - Enfermedades y plagas
K1 Phytopathologie
K1 Synergism
K1 Antifungal
K1 Fungicides
K1 Table grapes
K1 Uvas - Variedades
K1 Uvas - Enfermedades y plagas
K1 Grapes - Diseases and pests
K1 Ecology
K1 Plant science
K1 2414.06 Hongos
K1 3108.01 Bacterias
K1 3108 Fitopatología
K1 3101.05 Fungicidas
K1 2417.13 Ecología Vegetal
K1 3309 Tecnología de Los Alimentos
AB Botrytis cinerea is the most harmful postharvest disease of table grapes. Among the strategies that can be envisaged for its control, the use of coatings based on natural products is particularly promising. The study presented herein focuses on the assessment of the antagonistic capacity of two Streptomyces species and their culture filtrates against B. cinerea. Firstly, the secondary metabolites were characterized by gas chromatography–mass spectrometry, with N1-(4-hydroxybutyl)-N3-methylguanidine acetate and 2R,3S-9-[1,3,4-trihydroxy-2-butoxymethyl]guanine acetate as the main compounds produced by S. lavendofoliae DSM 40217; and cyclo(leucyloprolyl) and cyclo(phenylalanylprolyl) as the most abundant chemical species for S. rochei DSM 41729. Subsequently, the capacity of S. lavendofoliae DSM 40217 and S. rochei DSM 41729 to inhibit the growth of the pathogen was tested in dual culture plate assays, finding 85–90% inhibition. In agar dilution tests, their culture filtrates resulted in effective concentration values (EC90) in the 246–3013 μg·mL−1 range. Upon the formation of conjugate complexes with chitosan oligomers (COS) to improve solubility and bioavailability, a synergistic behavior was observed, resulting in lower EC90 values, ranging from 201 to 953 μg·mL−1. Ex situ tests carried out on ‘Timpson’ and ‘Red Globe’ table grapes using the conjugate complexes as coatings were found to maintain the turgor of the grapes and delay the appearance of the pathogen by 10−15 days at concentrations in the 750−1000 µg·mL−1 range. Hence, the conjugate complexes of COS and the selected Streptomyces spp. culture filtrates may be put forward as promising protection treatments for the sustainable control of gray mold.
PB MDPI
SN 2223-7747
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/69449
UL https://uvadoc.uva.es/handle/10324/69449
LA eng
NO Plants, 2023, Vol. 12, Nº. 3, 577
NO Producción Científica
DS UVaDOC
RD 10-nov-2024