RT info:eu-repo/semantics/article
T1 Antifungal activity of plant waste extracts against phytopathogenic fungi: Allium sativum peels extract as a promising product targeting the fungal plasma membrane and cell wall
A1 Teixeira, Ana
A1 Sánchez Hernández, Eva
A1 Noversa, João
A1 Cunha, Ana
A1 Cortez, Isabel
A1 Marques, Guilhermina
A1 Martín Ramos, Pablo
A1 Oliveira, Rui
K1 Agricultural wastes
K1 Residuos agrícolas
K1 Waste management
K1 Residuos agrícolas - Reciclaje - Aspecto del Medio Ambiente
K1 Residuos - Aprovechamiento
K1 Antifungal activity
K1 Fungicides
K1 Phytopathogenic fungi
K1 Hongos fitopatógenos
K1 Fungi
K1 Garlic
K1 Plant extracts
K1 Environmental protection
K1 Medio ambiente- - Protección
K1 Horticulture
K1 Plant science
K1 3102 Ingeniería Agrícola
K1 2414.90 Degradación de Residuos Vegetales
K1 3101.05 Fungicidas
K1 3108.05 Hongos
K1 3107 Horticultura
AB The harmful effect of synthetic fungicides on the environment and the development of resistance by fungi raises concerns about their security and future efficacy. In this work, we investigated plant by-products with the antifungal activity that could be safe alternatives to conventional fungicides. The in vitro antifungal potential of plant by-product extracts showed that garlic peel extract (GPE) was the most effective against several phytopathogenic fungi. Accordingly, in ex situ assays with apples, GPE significantly reduced the lesion size caused by subepidermal inoculation with Colletotrichum acutatum spores. In addition, Saccharomyces cerevisiae mutant strains affected in ergosterol synthesis showed higher resistance to GPE than the parental strain, indicating that the extract might target an intermediate of this pathway. Moreover, GPE affects the cell wall, given that bck1 and mkk1/mkk2 mutants were less able to cope with the stress because of the impairment of the remodeling mechanisms. Regarding the apoptosis-deficient mutant yca1, sensitivity was similar to that of the parental strain, suggesting that the extract does not induce apoptosis. A diverse group of sulfur compounds was identified by gas chromatography–mass spectrometry (GC/MS). Our findings contribute to the elucidation of the antifungal mechanism of GPE and highlight its potential as an alternative biofungicide in agriculture.
PB MDPI
SN 2311-7524
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/69517
UL https://uvadoc.uva.es/handle/10324/69517
LA eng
NO Horticulturae, 2023, Vol. 9, Nº. 2, 136
NO Producción Científica
DS UVaDOC
RD 10-nov-2024