RT info:eu-repo/semantics/article
T1 Protective actions of α-tocopherol on cell membrane lipids of paraquat-stressed human astrocytes using microarray technology, MALDI-MS and lipidomic analysis
A1 Sánchez Sánchez, Laura
A1 Fernández, Roberto
A1 Ganfornina Álvarez, María Dolores
A1 Astigarraga, Egoitz
A1 Barreda Gómez, Gabriel
K1 Oxidative stress
K1 Estrés oxidativo
K1 Lipids
K1 Lípidos
K1 Lipids - Peroxidation
K1 Microarrays
K1 DNA microarrays
K1 ADN
K1 Cells - Aging
K1 Aging
K1 Envejecimiento
K1 Astrocytes
K1 Astrocitos
K1 Genetics
K1 Genética
K1 Biochemistry
K1 Clinical biochemistry
K1 Bioquímica clínica
K1 Cell biology
K1 2302 Bioquímica
K1 2407 Biología Celular
AB Cellular senescence is one of the main contributors to some neurodegenerative disorders. The early detection of senescent cells or their related effects is a key aspect in treating disease progression. In this functional deterioration, oxidative stress and lipid peroxidation play an important role. Endogenous antioxidant compounds, such as α-tocopherol (vitamin E), can mitigate these undesirable effects, particularly lipid peroxidation, by blocking the reaction between free radicals and unsaturated fatty acid. While the antioxidant actions of α-tocopherol have been studied in various systems, monitoring the specific effects on cell membrane lipids at scales compatible with large screenings has not yet been accomplished. Understanding the changes responsible for this protection against one of the consequences of senescence is therefore necessary. Thus, the goal of this study was to determinate the changes in the lipid environment of a Paraquat-treated human astrocytic cell line, as a cellular oxidative stress model, and the specific actions of the antioxidant, α-tocopherol, using cell membrane microarray technology, MALDI-MS and lipidomic analysis. The stress induced by Paraquat exposure significantly decreased cell viability and triggered membrane lipid changes, such as an increase in certain species of ceramides that are lipid mediators of apoptotic pathways. The pre-treatment of cells with α-tocopherol mitigated these effects, enhancing cell viability and modulating the lipid profile in Paraquat-treated astrocytes. These results demonstrate the lipid modulation effects of α-tocopherol against Paraquat-promoted oxidative stress and validate a novel analytical high-throughput method combining cell cultures, microarray technology, MALDI-MS and multivariate analysis to study antioxidant compounds against cellular senescence.
PB MDPI
SN 2076-3921
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/69615
UL https://uvadoc.uva.es/handle/10324/69615
LA eng
NO Antioxidants, 2022, Vol. 11, Nº. 12, 2440
NO Producción Científica
DS UVaDOC
RD 14-oct-2024