RT info:eu-repo/semantics/article
T1 Valorization of Quercus suber L. bark as a source of phytochemicals with antimicrobial activity against apple tree diseases
A1 Sánchez Hernández, Eva
A1 González García, Vicente
A1 Casanova Gascón, José
A1 Barriuso Vargas, Juan José
A1 Balduque Gil, Joaquín
A1 Lorenzo Vidal, Belén
A1 Martín Gil, Jesús
A1 Martín Ramos, Pablo
K1 Antimicrobial activity
K1 Antifungal
K1 Ecology
K1 Phytochemicals
K1 Fitoquímica
K1 Phytopathologie
K1 Trees - Diseases and pests
K1 Arboles - Enfermedades y plagas
K1 Alcornoques
K1 Apples
K1 Manzanos - Enfermedades y plagas
K1 Forests and forestry
K1 Bosques y silvicultura
K1 Plant science
K1 Fruit-culture
K1 2417.13 Ecología Vegetal
K1 3108 Fitopatología
K1 3102 Ingeniería Agrícola
K1 3106.08 Silvicultura
K1 3107.04 Fruticultura
AB Cork, an anatomic adaptation of the bark of Quercus suber L. through its suberization process, finds its main application in the production of bottle stoppers. Its processing results in a large waste stream of cork fragments, granulates, and dust, which may be susceptible to valorization. The work presented here explored the use of its extracts to inhibit the growth of phytopathogenic microorganisms associated with apple tree diseases. The in vitro antimicrobial activity of cork aqueous ammonia extract was assayed against four fungi, viz. Monilinia fructigena and M. laxa (brown rot), Neofussicoccum parvum (dieback), and Phytophthora cactorum (collar and root rot), and two bacteria, viz. Erwinia amylovora and Pseudomonas syringae pv. syringae, either alone or in combination with chitosan oligomers (COS). Effective concentration values of EC90 in the 675–3450 μg·mL−1 range, depending on the fungal pathogen, were obtained in growth inhibition tests, which were substantially improved for the conjugate complexes (340–801 μg·mL−1) as a result of strong synergism with COS. Similar enhanced behavior was also observed in antibacterial activity assays, with MIC values of 375 and 750 μg·mL−1 for the conjugate complexes against P. syringae pv. syringae and E. amylovora, respectively. This in vitro inhibitory activity was substantially higher than those exhibited by azoxystrobin and fosetyl-Al, which were tested for comparison purposes, and stood out among those reported for other natural compounds in the literature. The observed antimicrobial activity may be mainly attributed to the presence of glycerin and vanillic acid, identified by gas chromatography–mass spectroscopy. In the first step towards in-field application, the COS–Q. suber bark extract conjugate complex was further tested ex situ against P. cactorum on artificially inoculated excised stems of the ‘Garnem’ almond rootstock, achieving high protection at a dose of 3750 μg·mL−1. These results suggest that cork industrial leftovers may, thus, be a promising source of bioactive compounds for integrated pest management.
PB MDPI
SN 2223-7747
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/69621
UL https://uvadoc.uva.es/handle/10324/69621
LA eng
NO Plants, 2022, Vol. 11, Nº. 24, 3415
NO Producción Científica
DS UVaDOC
RD 18-nov-2024