RT info:eu-repo/semantics/article T1 Bio-2,3-butanediol production from banana waste: Preliminary techno-economic evaluation of processing strategies A1 Fernández-Delgado, Marina A1 Rodríguez-Sarmiento, Mercedes A1 Coral Medina, Jesús David A1 Lucas, Susana A1 García-Cubero, Mª Teresa A1 Coca, Mónica A1 López-Linares, Juan Carlos K1 2,3-Butanediol; banana waste; Sustainable bioprocessing; K1 Simultaneous saccharification and fermentation (SSF); Sequential hydrolysis and fermentation (SHF); Economic analysis AB This study evaluates different fermentation strategies to produce 2,3-butanediol (2,3-BD) from banana industrywaste, such as whole bananas (fruit + peels) and banana peels, selecting the most favorable from a technical andeconomic point of view. Both residues have enough free sugars (17.8 %–35.8 %), glucan (11.0 %–14.2 %) andhemicellulose (2.8 %–6.3 %), to be promising substrates for 2,3-BD fermentation. Saccharification was studied bycomparing enzymatic hydrolysis, hydrothermal pretreatment, and hydrothermal pretreatment followed byenzymatic hydrolysis. Different fermentation scenarios were also compared regarding the 2,3-BD yield andproductivity: Separate Hydrolysis and Fermentation (SHF), Simultaneous Saccharification and Fermentation(SSF), and direct fermentation without prior saccharification using Paenibacillus polymyxa DSM-365 as the fermenting microorganism. The results showed that the pretreatment step was not necessary to improve the release of fermentable sugars. Enzymatic hydrolysis was the most effective alternative for maximizing sugar recovery, reaching sugar concentrations of 18.1 g/L (recovery: 92.5 %) for banana peels and 33.3 g/L (recovery: ~100 %) for whole bananas. The SSF strategy led to higher 2,3-BD concentrations of 15.0 g/L and 26.6 g/L for banana peels and whole bananas, respectively. The preliminary economic analysis indicated that SSF and direct fermentation could be the more cost-effective process alternatives for banana peels and whole bananas, respectively. Thus, it was demonstrated that banana waste is an interesting resource for the production of 2,3-BD. The bioprocess can be competitive when using a low-cost raw material and reducing the number of process steps compared to traditional technologies. PB Elsevier YR 2024 FD 2024 LK https://uvadoc.uva.es/handle/10324/70446 UL https://uvadoc.uva.es/handle/10324/70446 LA eng NO Biomass and Bioenergy 184, 107218 DS UVaDOC RD 10-nov-2024