RT dataset T1 Recovering rhamnogalacturonan-I pectin from sugar beet pulp using a sequential ultrasound and microwave-assisted extraction: Study on extraction optimization and membrane purification (dataset) A1 Amo Mateos, Esther Del A1 Cáceres, Berta A1 Coca Sanz, Mónica A1 García Cubero, María Teresa A1 Lucas Yagüe, Susana A2 Universidad de ValladolidInstituto de Procesos Sostenibles K1 Pectooligosaccharides; Box-Behnken design; Green extraction; ultrafiltration; diafiltration AB This research focuses on the integrated recovery of rhamnogalacturonan-I (RG-I) pectin from sugar beet pulp (SBP). First, the extraction of RG-I pectin through sequential ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) was assessed. Optimization using a response surface methodology identified the optimal conditions as initial pH 4, 10 minutes of UAE, and 157 °C for MAE, achieving a 66.0 % recovery of pectooligosaccharides (POS). Additionally, purification through continuous diafiltration and concentration via ultrafiltration of the POS using membranes with different molecular weight cut-offs (MWCO) was explored. In contrast to previous research using discontinuous diafiltration, the use of continuous diafiltration allowed a decrease in the extract viscosity and obtained higher yields using a higher MWCO membrane. The refined RG-I pectin solids exhibited a high global yield (39 – 40 g pectin/100 g SBP), and high-methoxyl characteristics, as well as purity levels (70 – 80 %) similar to commercial prebiotics. YR 2024 FD 2024 LK https://uvadoc.uva.es/handle/10324/70814 UL https://uvadoc.uva.es/handle/10324/70814 LA eng NO Bioresource Technology, 2024, 394, 130263. NO Instituto de Procesos Sostenibles DS UVaDOC RD 02-abr-2025