RT info:eu-repo/semantics/masterThesis T1 Producción sostenible de péptidos y PHAS a partir de biomasa crecida en fotobiorreactores de tratamiento de aguas residuales A1 Gómez Calderón, Belén A2 Universidad de Valladolid. Escuela de Ingenierías Industriales K1 Bioplásticos K1 Carbohidratos K1 Microalgas K1 Proteínas K1 Purines de cerdo K1 Recuperación K1 Valorización K1 3303 Ingeniería y Tecnología Químicas AB This Work presents a preliminary experimental study of bioplastic production and peptide recovery from microalgae and bacteria biomass, grown in pig purine treatment photobioreactors. The biomass has been hydrolyzed by different methods, obtaining the highest solubilizations with acid and alkaline hydrolysis at 121ºC (~15 g/L of TOC and 4 g/L of TN), approximately double that with enzymatic hydrolysis assisted with ultrasound. The best growth results of the microorganism Paracoccus denitrificans, an organism capable of accumulating polyhydroxyalkanoates, were obtained with hydrolyzed acid diluted to 10% (1.68 g/L). The degradation compounds generated in the chemical hydrolyses inhibited the growth of the microorganism in alkaline hydrolyzate. The enzymatic hydrolyzate provided lower concentrations of microorganisms (0.95 g/L) but larger peptides, which could be separated by different techniques. Approximately 50% of the peptides in the enzymatic hydrolyzate were precipitated with acetone, ethanol, and ammonium sulfate. YR 2024 FD 2024 LK https://uvadoc.uva.es/handle/10324/71201 UL https://uvadoc.uva.es/handle/10324/71201 LA spa NO Departamento de Ingeniería Química y Tecnología del Medio Ambiente DS UVaDOC RD 23-abr-2025