RT info:eu-repo/semantics/article
T1 Human Mesenchymal Stem Cell Secretome Exhibits a Neuroprotective Effect over In Vitro Retinal Photoreceptor Degeneration
A1 Usategui Martín, Ricardo
A1 Puertas Neyra, Kevin Louis
A1 García Gutiérrez, María Teresa
A1 Fuentes, Manuel
A1 Pastor Jimeno, José Carlos
A1 Fernández Bueno, Iván
AB Retinal photoreceptor degeneration occurs frequently inseveral neurodegenerative retinal diseases such as age-relatedmacular degeneration, retinitis pigmentosa, or genetic retinaldiseases related to the photoreceptors. Despite the impact ondaily life and the social and economic consequences, there isno cure for these diseases. Considering this, cell-based therapymay be an optimal therapeutic option. This study evaluated theneuroprotective in vitro potential of a secretome of humanbone marrow mesenchymal stem cells (MSCs) for retinal pho-toreceptors in vitro. We analyzed the photoreceptor morpho-logic changes and the paracrine factors secreted by humanbone marrow MSCs in a physically separated co-culture withdegenerated neuroretinas, using organotypic neuroretinalcultures. The results showed that the secretome of humanbone marrow MSCs had a neuroprotective effect over theneuroretinal general organization and neuropreserved thephotoreceptors from degeneration probably by secretion ofneuroprotective proteins. The study of the expression of1,000 proteins showed increased paracrine factors secreted byMSCs that could be crucial in the neuroprotective effect ofthe stem cell secretome over in vitro retinal degeneration.The current results reinforce the hypothesis that the paracrineeffect of the human bone marrow MSCs may slow photore-ceptor neurodegeneration and be a therapeutic option inretinal photoreceptor degenerative diseases.
SN 2329-0501
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/71471
UL https://uvadoc.uva.es/handle/10324/71471
LA eng
NO Molecular Therapy - Methods & Clinical Development 2020;17:1155-1166
NO Producción Científica
DS UVaDOC
RD 14-ene-2025