RT info:eu-repo/semantics/article
T1 Evidence for two types of nicotinic receptors in the cat carotid body chemoreceptor cells
A1 Obeso Cáceres, Ana María de la Luz
A1 Gómez Niño, María Ángeles
A1 Almaraz Gómez, Laura
A1 Dinger, Bruce
A1 Fidone, Salvatore
A1 González, Constancio
K1 Neurofisiología
K1 Receptores celulares
AB Current concepts on the location and functional significance of nicotinic receptors in the carotid body rest on a-bungarotoxin bindingand autoradiographic studies. Using an in vitro preparation of the cat carotid body whose catecholamine deposits have been labeled byprior incubation with the tritiated natural precursor w3Hxtyrosine, we have found that nicotine induces release of w3Hxcatecholamines in adose-dependent manner  IC50s9.81 mM.. We also found that mecamylamine  50 mM. completely abolished the nicotine-inducedrelease, while a-bungarotoxin  100 nM; f20 times its binding Kd. only reduced the release by 56%. These findings indicate thatchemoreceptor cells, and perhaps other carotid body structures, contain nicotinic receptors that are not sensitive to a-bungarotoxin andforce a revision of the current concepts on cholinergic mechanisms in the carotid body chemoreception.
PB Elsevier
SN 0006-8993
YR 1997
FD 1997
LK http://uvadoc.uva.es/handle/10324/7158
UL http://uvadoc.uva.es/handle/10324/7158
LA eng
NO Brain Research 754  1997. 298–302
NO Producción Científica
DS UVaDOC
RD 24-nov-2024