RT info:eu-repo/semantics/bookPart
T1 Optimization of human limbal stem cell culture by replating a single limbal explant
A1 López Paniagua, Marina
A1 Nieto-Miguel, Teresa
A1 Galindo de la Rosa, Sara
A1 García Posadas, Laura
A1 De La Mata Sampedro, Ana
A1 Corrales, Rosa María
A1 Diebold Luque, María Yolanda
K1 Limbo
K1 Células madre
K1 Cornea
K1 Corneal epithelium
K1 Corneoscleral samples
K1 Limbus
K1 Limbal explant
K1 Limbal stem cells
K1 Limbal primary cultures
K1 In vitro expansion
K1 2407 Biología Celular
AB Cultured limbal epithelial stem cell transplantation is a clinical procedure used to regenerate the cornealepithelium in patients with limbal stem cell deficiency. The protocols used to expand limbal epithelial cellsin vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it is possible to obtain up to three limbal epithelial primary cultures from the same explant, thus increasing the efficiency of the in vitro cell culture.
PB Springer
SN 1064-3745
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/71681
UL https://uvadoc.uva.es/handle/10324/71681
LA eng
NO López-Paniagua M, Nieto-Miguel T, Galindo S, García-Posadas L, de la Mata A, Corrales RM, Calonge M, Diebold Y. Methods in molecular biology. Nueva York: Springer, 2020, vol. 2145, p. 39  p. 39-50.
NO Producción Científica
DS UVaDOC
RD 23-nov-2024