RT info:eu-repo/semantics/article
T1 Influence of the Molecular Weight on the Inverse Temperature Transition of a Model Genetically Engineered Elastin-like pH-Responsive Polymer
A1 Girotti, Alessandra
A1 Reguera, Javier
A1 Arias Vallejo, Francisco Javier
A1 Alonso Rodrigo, Matilde
A1 Testera Gorgojo, Ana María
A1 Rodríguez Cabello, José Carlos
AB The pH-responsive elastin-like polymers, [(PGVGV)2−(PGEGV)−(PGVGV)2]n with n = 5, 9, 15, 30, 45, were obtained using genetic engineering and microbial protein expression. These were intended to study the effects of the molecular weight (MW) on the properties of their inverse temperature transition (ITT) and its dependence on pH. As a result, the transition temperature decreased and the transition enthalpy increased as the molecular weight increased, especially for the lowest MWs. More strikingly, the apparent pKa for the γ-carboxyl residue of the glutamic acid also depends on MW. The apparent pKa is lower for lower MWs. In summary, the modification in the ITT caused by changes in MW is similar to the one caused by changes in the mean polarity of the polymer as described in the literature. A reduction in the molecular weight is equivalent to a decrease in the mean hydrophobicity of the polymer.
YR 2004
FD 2004
LK https://uvadoc.uva.es/handle/10324/72536
UL https://uvadoc.uva.es/handle/10324/72536
LA eng
NO Macromolecules  37, 3396-3400 (2004)
NO Producción Científica
DS UVaDOC
RD 23-dic-2024