RT info:eu-repo/semantics/article
T1 Leptin receptors in human skeletal muscle
A1 Guerra, Borja
A1 Santana, Alfredo
A1 Fuentes Nieto, Teresa
A1 Delgado Guerra, Safira
A1 Cabrera Socorro, Alfredo
A1 Dorado, Cecilia
A1 Calbet, Jose A. L.
AB Human skeletalmuscle expresses leptin receptor mRNA; however, it remains un-known whether leptin receptors (OB-R) are also expressed at theprotein level. Fourteen healthy men (age   33.1   2.0 yr, height  175.9   1.7 cm, body mass   81.2   3.8 kg, body fat   22.5  1.9%; means   SE) participated in this investigation. The expressionof OB-R protein was determined in skeletal muscle, subcutaneousadipose tissue, and hypothalamus using a polyclonal rabbit anti-human leptin receptor. Three bands with a molecular mass close to170, 128, and 98 kDa were identified by Western blot with theanti-OB-R antibody. All three bands were identified in skeletal muscle:the 98-kDa and 170-kDa bands were detected in hypothalamus, and the98-kDa and 128-kDa bands were detected in thigh subcutaneous adiposetissue. The 128-kDa isoform was not detected in four subjects,whereas in the rest its occurrence was fully explained by the presenceof intermuscular adipose tissue, as demonstrated using an anti-perili-pin A antibody. No relationship was observed between the basalconcentration of leptin in serum and the 170-kDa band density. Inconclusion, a long isoform of the leptin receptor with a molecularmass close to 170 kDa is expressed at the protein level in humanskeletal muscle. The amount of 170-kDa protein appears to beindependent of the basal concentration of leptin in serum.
PB American Physiological Society
SN 8750-7587
YR 2007
FD 2007
LK https://uvadoc.uva.es/handle/10324/72600
UL https://uvadoc.uva.es/handle/10324/72600
LA eng
NO Journal of Applied Physiology, 2007, vol 102, n. 5, p. 1786-1792
DS UVaDOC
RD 02-abr-2025