RT info:eu-repo/semantics/article
T1 Leptin receptors in human skeletal muscle
A1 Guerra, Borja
A1 Santana, Alfredo
A1 Fuentes, Teresa
A1 Delgado-Guerra, Safira
A1 Cabrera-Socorro, Alfredo
A1 Dorado, Cecilia
A1 Calbet, Jose A. L.
AB Human skeletalmuscle expresses leptin receptor mRNA; however, it remains un-known whether leptin receptors (OB-R) are also expressed at theprotein level. Fourteen healthy men (age   33.1   2.0 yr, height  175.9   1.7 cm, body mass   81.2   3.8 kg, body fat   22.5  1.9%; means   SE) participated in this investigation. The expressionof OB-R protein was determined in skeletal muscle, subcutaneousadipose tissue, and hypothalamus using a polyclonal rabbit anti-human leptin receptor. Three bands with a molecular mass close to170, 128, and 98 kDa were identified by Western blot with theanti-OB-R antibody. All three bands were identified in skeletal muscle:the 98-kDa and 170-kDa bands were detected in hypothalamus, and the98-kDa and 128-kDa bands were detected in thigh subcutaneous adiposetissue. The 128-kDa isoform was not detected in four subjects,whereas in the rest its occurrence was fully explained by the presenceof intermuscular adipose tissue, as demonstrated using an anti-perili-pin A antibody. No relationship was observed between the basalconcentration of leptin in serum and the 170-kDa band density. Inconclusion, a long isoform of the leptin receptor with a molecularmass close to 170 kDa is expressed at the protein level in humanskeletal muscle. The amount of 170-kDa protein appears to beindependent of the basal concentration of leptin in serum.
PB J Appl Physiol
SN 8750-7587
YR 2007
FD 2007
LK https://uvadoc.uva.es/handle/10324/72600
UL https://uvadoc.uva.es/handle/10324/72600
LA spa
NO Guerra B, Santana A, Fuentes T, Delgado-Guerra S, Cabrera-Socorro A, Dorado C, Calbet JA. Leptin receptors in human skeletal muscle. J Appl Physiol (1985). 2007 May;102(5):1786-92. doi: 10.1152/japplphysiol.01313.2006. Epub 2007 Jan 18. PMID: 17234799.
DS UVaDOC
RD 21-dic-2024