RT info:eu-repo/semantics/article
T1 Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity
A1 Guerra, Borja
A1 Fuentes Nieto, Teresa
A1 Delgado Guerra, Safira
A1 Guadalupe Grau, Amelia
A1 Olmedillas, Hugo
A1 Santana, Alfredo
A1 Ponce González, Jesús Gustavo
A1 Dorado, Cecilia
A1 Calbet, José A. L.
AB To determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) and the protein suppressor of cytokine signaling 3 (SOCS3) in human skeletal muscle, the protein expression of OB-R, perilipin A, SOCS3 and alpha-tubulin was assessed by Western blot in muscle biopsies obtained from the m. vastus lateralis in thirty-four men (age = 27.1±6.8 yr) and thirty-three women (age = 26.7±6.7 yr). Basal serum insulin concentration and HOMA were similar in both genders. Serum leptin concentration was 3.4 times higher in women compared to men (P<0.05) and this difference remained significant after accounting for the differences in percentage of body fat or soluble leptin receptor. OB-R protein was 41% (OB-R170, P<0.05) and 163% (OB-R128, P<0.05) greater in women than men. There was no relationship between OB-R expression and the serum concentrations of leptin or 17β-estradiol. In men, muscle OB-R128 protein was inversely related to serum free testosterone. In women, OB-R98 and OB-R128 were inversely related to total serum testosterone concentration, and OB-R128 to serum free testosterone concentration. SOCS3 protein expression was similar in men and women and was not related to OB-R. In women, there was an inverse relationship between the logarithm of free testosterone and SCOS3 protein content in skeletal muscle (r = −0.46, P<0.05). In summary, there is a gender dimorphism in skeletal muscle leptin receptors expression, which can be partly explained by the influence of testosterone. SOCS3 expression in skeletal muscle is not up-regulated in women, despite very high serum leptin concentrations compared to men. The circulating form of the leptin receptor can not be used as a surrogate measure of the amount of leptin receptors expressed in skeletal muscles.
PB PLoS ONE
YR 2008
FD 2008-10
LK https://uvadoc.uva.es/handle/10324/72605
UL https://uvadoc.uva.es/handle/10324/72605
LA eng
NO PLoS One, 2008, vol. 3, n. 10, e3466
NO Producción Científica
DS UVaDOC
RD 04-abr-2025