RT info:eu-repo/semantics/article
T1 Gender Dimorphism in Skeletal Muscle Leptin Receptors, Serum Leptin and Insulin Sensitivity
A1 Guerra, Borja
A1 Fuentes, Teresa
A1 Delgado-Guerra, Safira
A1 Guadalupe-Grau, Amelia
A1 Olmedillas, Hugo
A1 Santana, Alfredo
A1 Ponce-Gonzalez, Jesus Gustavo
A1 Dorado, Cecilia
A1 Calbet, José A. L.
AB Todetermineifthereisagenderdimorphismintheexpressionof leptinreceptors(OB-R170,OB-R128andOB-R98)andthe proteinsuppressorofcytokinesignaling3(SOCS3) inhumanskeletalmuscle, theproteinexpressionofOB-R,perilipinA, SOCS3andalpha-tubulinwasassessedbyWesternblot inmusclebiopsiesobtainedfromthem.vastus lateralis inthirtyfourmen(age=27.166.8yr) andthirty-threewomen(age=26.766.7yr). Basal seruminsulinconcentrationandHOMA weresimilar inbothgenders.Serumleptinconcentrationwas3.4timeshigher inwomencomparedtomen(P,0.05)and thisdifferenceremainedsignificantafteraccountingforthedifferencesinpercentageofbodyfatorsolubleleptinreceptor. OB-Rproteinwas 41%(OB-R170, P,0.05) and163%(OB-R128, P,0.05) greater inwomen thanmen. Therewas no relationshipbetweenOB-Rexpressionandtheserumconcentrationsof leptinor17b-estradiol. Inmen,muscleOB-R128 proteinwas inverselyrelatedtoserumfreetestosterone. Inwomen,OB-R98andOB-R128wereinverselyrelatedtototal serumtestosteroneconcentration,andOB-R128toserumfreetestosteroneconcentration.SOCS3proteinexpressionwas similarinmenandwomenandwasnotrelatedtoOB-R. Inwomen,therewasaninverserelationshipbetweenthelogarithm of free testosteroneandSCOS3proteincontent inskeletalmuscle (r=20.46, P,0.05). Insummary, there is agender dimorphisminskeletalmuscleleptinreceptorsexpression,whichcanbepartlyexplainedbytheinfluenceoftestosterone. SOCS3 expression in skeletal muscle is not up-regulated inwomen, despite very high serum leptin concentrations comparedtomen.Thecirculatingformof theleptinreceptorcannotbeusedasasurrogatemeasureof theamountof leptinreceptorsexpressedinskeletalmuscles.
PB PLoS ONE
YR 2008
FD 2008-10
LK https://uvadoc.uva.es/handle/10324/72605
UL https://uvadoc.uva.es/handle/10324/72605
LA eng
NO Guerra B, Fuentes T, Delgado-Guerra S, Guadalupe-Grau A, Olmedillas H, Santana A, Ponce-Gonzalez JG, Dorado C, Calbet JA. Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. PLoS One. 2008;3(10):e3466. doi: 10.1371/journal.pone.0003466. Epub 2008 Oct 21. PMID: 18941624; PMCID: PMC2565105.
NO Producción Científica
DS UVaDOC
RD 21-dic-2024