RT info:eu-repo/semantics/article
T1 SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion
A1 Guerra, Borja
A1 Guadalupe-Grau, Amelia
A1 Fuentes, Teresa
A1 Ponce-González, Jesús Gustavo
A1 Morales-Alamo, David
A1 Olmedillas, Hugo
A1 Guillén-Salgado, José
A1 Santana, Alfredo
A1 Calbet, José A. L.
AB This study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr172-AMPK phosphorylation and increased PGC-1α mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKα phosphorylation. Exercise elicited different patterns of Ser221-ACCβ, Ser473-Akt and Thr642-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation was reduced by 33% coinciding with increased Thr172-AMPKα phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr172-AMPKα phosphorylation. Ser221-ACCβ phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr172-AMPKα and Ser221-ACCβ phosphorylation. Two hours after the sprint, PGC-1α protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1α protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr172-AMPKα phosphorylation and its downstream signaling during the recovery period.
SN 1439-6319
YR 2010
FD 2010-03
LK https://uvadoc.uva.es/handle/10324/72607
UL https://uvadoc.uva.es/handle/10324/72607
LA eng
NO Guerra B, Guadalupe-Grau A, Fuentes T, Ponce-González JG, Morales-Alamo D, Olmedillas H, Guillén-Salgado J, Santana A, Calbet JA. SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion. Eur J Appl Physiol. 2010 Jul;109(4):731-43. doi: 10.1007/s00421-010-1413-y. Epub 2010 Mar 9. PMID: 20217115.
NO Producción Científica
DS UVaDOC
RD 20-dic-2024