RT info:eu-repo/semantics/article
T1 SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion
A1 Guerra, Borja
A1 Guadalupe Grau, Amelia
A1 Fuentes Nieto, Teresa
A1 Ponce González, Jesús Gustavo
A1 Morales Álamo, David
A1 Olmedillas, Hugo
A1 Guillén Salgado, José
A1 Santana, Alfredo
A1 Calbet, José A. L.
AB This study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr172-AMPK phosphorylation and increased PGC-1α mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKα phosphorylation. Exercise elicited different patterns of Ser221-ACCβ, Ser473-Akt and Thr642-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation was reduced by 33% coinciding with increased Thr172-AMPKα phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr172-AMPKα phosphorylation. Ser221-ACCβ phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr172-AMPKα and Ser221-ACCβ phosphorylation. Two hours after the sprint, PGC-1α protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1α protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr172-AMPKα phosphorylation and its downstream signaling during the recovery period.
SN 1439-6319
YR 2010
FD 2010-03
LK https://uvadoc.uva.es/handle/10324/72607
UL https://uvadoc.uva.es/handle/10324/72607
LA eng
NO European Journal of Applied Physiology, 2010, vol. 109, n. 4, p. 731-743
NO Producción Científica
DS UVaDOC
RD 21-jun-2025