RT info:eu-repo/semantics/article
T1 Leptin signaling in skeletal muscle after bed rest in healthy humans
A1 Guerra, Borja
A1 Ponce González, Jesús Gustavo
A1 Morales Álamo, David
A1 Guadalupe Grau, Amelia
A1 Kiilerich, Kristian
A1 Fuentes Nieto, Teresa
A1 Ringholm, Stine
A1 Biensø, Rasmus Sjørup
A1 Santana, Alfredo
A1 Lundby, Carsten
A1 Pilegaard, Henriette
A1 Calbet, José A. L.
AB This study aimed at determining the effects of bed rest on the skeletal muscle leptin signaling system. Deltoid and vastus lateralis muscle biopsies and blood samples were obtained from 12 healthy young men (mean ± SD, BMI 22.8 ± 2.7 kg/m(2)) before and after 7 days of bed rest. Leptin receptor isoforms (OB-Rs), suppressor of cytokine signaling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) protein expression and signal transducer and activator of transcription 3 (STAT3) phosphorylation were analyzed by Western blot. After bed rest basal insulin concentration was increased by 53 % (P < 0.05), the homeostasis model assessment (HOMA) by 40 % (P < 0.05), and serum leptin concentration by 35 % (P < 0.05) with no changes in body fat mass. Although the soluble isoform of the leptin receptor (s-OBR) remained unchanged, the molar excess of leptin over sOB-R was increased by 1.4-fold after bed rest (P < 0.05). OB-Rs and SOCS3 protein expression, and STAT3 phosphorylation level remained unaffected in deltoid and vastus lateralis by bed rest, as PTP1B in the deltoid. PTP1B was increased by 90 % with bed rest in the vastus lateralis (P < 0.05). There was a linear relationship between the increase in vastus lateralis PTP1B and the increase in both basal insulin concentrations (r = 0.66, P < 0.05) and HOMA (r = 0.68, P < 0.05) with bed rest. One week of bed rest is associated with increased leptin levels without augmenting STAT3 phosphorylation indicating some degree of leptin resistance in skeletal muscle, which can be explained, at least in part, by an elevation of PTP1B protein content in the vastus lateralis muscle.
PB Springer
SN 1439-6319
YR 2014
FD 2014-02
LK https://uvadoc.uva.es/handle/10324/72645
UL https://uvadoc.uva.es/handle/10324/72645
LA eng
NO Guerra B, Ponce-González JG, Morales-Alamo D, Guadalupe-Grau A, Kiilerich K, Fuentes T, Ringholm S, Biensø RS, Santana A, Lundby C, Pilegaard H, Calbet JA. Leptin signaling in skeletal muscle after bed rest in healthy humans. Eur J Appl Physiol. 2014 Feb;114(2):345-57. doi: 10.1007/s00421-013-2779-4. Epub 2013 Nov 29. PMID: 24292882.
NO Producción Científica
NO Ministerio de Ciencia e Innovación (BFU2006-13784 and FEDER) y FUncIS (PI/10/07).
DS UVaDOC
RD 04-abr-2025