RT info:eu-repo/semantics/article T1 Exploring new strategies for optimizing the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from methane and VFAs in synthetic cocultures and mixed methanotrophic consortia A1 Amabile, Claudia A1 Abate, Teresa A1 Marcos Naveira, José Enrique A1 Chianese, Simeone A1 Musmarra, Dino A1 Muñoz Torre, Raúl K1 Biopolymers K1 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) K1 Ustainable process K1 Mixed methanotrophic consortium K1 Synthetic coculture K1 Volatile fatty acid mixture K1 23 Química AB In this work, the potential of a synthetic cocultureand a mixed methanotrophic consortium to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from renewableand waste-based feedstocks was assessed batchwise. Methylocystisparvuscocultivated with Rhodococcus opacus and a Methylocystis-enriched culture previously grown on methane were subjected tonutrient starvation in a medium enriched with valeric acid (30% ww−1 of Ctot) or with a VFAs mixture containing acetic, propionic,butyric, and valeric acids (15% w w−1 of Ctot) under a CH4:O2 or airatmosphere. For all test series, pH was adjusted to 7 after addingthe cosubstrates, and a negligible substrate consumption or polymerproduction was considered the end point of the trial. Results showed that valeric acid promoted PHBV accumulation in bothcultures regardless of the atmosphere. Interestingly, the mixture of VFAs supported PHBV accumulation only in the presence ofmethane. The highest PHBV contents for the coculture and the mixed consortium, equal to 73.7 ± 2.5% w w−1 and 49.6 ± 13% ww−1, respectively, were obtained with methane and the VFAs mixture. This study demonstrates the suitability of cocultures andbiobased cosubstrates for the sustainable production of the biodegradable polymer PHBV. PB ACS (American Chemical Society) SN 2168-0485 YR 2024 FD 2024 LK https://uvadoc.uva.es/handle/10324/72661 UL https://uvadoc.uva.es/handle/10324/72661 LA eng NO ACS Sustainable Chemistry & Engineering, 2024, vol.12, n.11, p. 4690-4699 NO Producción Científica DS UVaDOC RD 18-dic-2024