RT info:eu-repo/semantics/article
T1 Three-sectioning method: A procedure for studying hard tissues and large pieces under light and electron microscopy
A1 Gayoso, Jorge
A1 Garrosa, Manuel
A1 Gayoso, Sara
A1 Rodríguez-Arias, Carlos Alberto
A1 Martin-Ferrero, Miguel Ángel
A1 Gayoso, Manuel José
K1 Hard Tissue, Prostheses, Plastic Enbedding, Epoxy resin, Sectioning, Bone, Nerve, Thick sections, Ultrathin sections, TEM, SEM.
AB The histological study of hard pieces such as tendons and calcified lesions and tissues is a field that has beengaining increased attention owing to the rapid development of implantable prostheses, among other factors. Inthese studies, serial sectioning is utilized to detect areas of interest throughout the entire piece, as it enables theapplication of the appropriate light and electron microscopy techniques in these areas. We propose the “threesectioningmethod” that subjects the pieces to three consecutive cycles of embedding and sectioning to localizeand study the areas of interest, as an efficient technique for these histological studies. The pieces were firstembedded in epoxy resin and then cut into thick sections (approximately 300 μm) for the first cycle. Next, areasof interest selected on these thick sections were re-embedded in epoxy resin to be sectioned again (secondsectioning) to obtain a series of semithin sections (1–3 μm). These semithin sections are usually studied using themost relevant techniques for light microscopy. Smaller areas of interest are selected to be cut into ultrathinsections (60–90 nm) for transmission electron microscopy. If necessary, the selected areas of the semithin sections an be embedded again, and then cut into new ultrathin sections. The different kinds of sections we have described here may also be studied using scanning electron microscopy. This systematic method facilitates correlative microscopy from lower to higher magnifications along with the usage of a broad variety of histological techniques including electron microscopy.
PB Elsevier
SN 0968-4328
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/72929
UL https://uvadoc.uva.es/handle/10324/72929
LA eng
NO Micron. May 2020 ;Vol.132:102841: p.1-6
NO Producción Científica
DS UVaDOC
RD 22-dic-2024