RT info:eu-repo/semantics/article T1 Biohydrogen production by lactate-driven dark fermentation of real organic wastes derived from solid waste treatment plants A1 Martínez Fraile, Cristina A1 Muñoz Torre, Raúl A1 Simorte, María Teresa A1 Sanz, Inmaculada A1 García Depraect, Octavio K1 Dark fermentation K1 Food waste K1 OFMSW K1 Leachate K1 Mixed sludge K1 hidro AB This study evaluated the hydrogen production potential through lactate-driven dark fermentation (LD-DF) of organic wastes from solid waste treatment plants, including the organic fraction of municipal solid waste (OFMSW), mixed sewage sludge, and two OFMSW leachates. In initial batch fermentations, only OFMSW supported a significant hydrogen yield (70.1 ± 7.7 NmL-H2/g-VS added) among the tested feedstocks. Lactate acted as an important hydrogen precursor, requiring the presence of carbohydrates for sequential two-step lactate-type fermentation. The impact of operational pH (5.5–6.5) and initial total solids (TS) concentration (5–12.5 % w/w) was also evaluated using OFMSW as substrate, obtaining hydrogen yields ranging from 6.6 to 55.9 NmL-H2/g-VSadded. The highest yield occurred at 6.5 pH and 7.5 % TS. The LD-DF pathway was indicated to be present under diverse pH and TS conditions, supported by employing a specialized microbial consortium capable of performing LD-DF, along with the observed changes in lactate levels during fermentation. PB Elsevier SN 0960-8524 YR 2024 FD 2024 LK https://uvadoc.uva.es/handle/10324/73065 UL https://uvadoc.uva.es/handle/10324/73065 LA eng NO Bioresource Technology, 2024, vol. 403, 130846 NO Producción Científica DS UVaDOC RD 05-feb-2025