RT info:eu-repo/semantics/article T1 Biomethanization of rigid packaging made entirely of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate): Mono- and co-digestion tests and microbial insights A1 García Depraect, Octavio A1 Martínez Mendoza, Leonardo José A1 Aragão Börner, Rosa A1 Zimmer, Johannes A1 Muñoz Torre, Raúl K1 Anaerobic digestion K1 Biodegradability K1 Bioplastics K1 Organic recycling K1 Packaging K1 PHBH AB This study evaluates the anaerobic mesophilic mono- and co-digestion of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) plastic bottles as a proxy for rigid packaging materials. Initial tests showed a 97.3 ± 0.2 % reduction in weight and an observable alteration in the surface (thinning, color fading and pitting) of the PHBH bottles after eight weeks. Subsequent tests showed that PHBH squares (3 × 3 cm) produced 400 NmL-CH4/g-VSfed, at a slower rate compared to powdered PHBH but with similar methane yield. Co-digestion experiments with food waste, swine manure, or sewage sludge showed successful digestion of PHBH alongside organic waste (even at a high bioplastic loading of 20 % volatile solids basis), with methane production comparable to or slightly higher than that observed in mono-digestion. Molecular analyses suggested that the type of co-substrate influenced microbial activity and that methane production was mainly driven by hydrogenotrophic methanogenesis. These results suggest the potential for integrating rigid PHBH packaging into anaerobic digesters. PB Elsevier SN 0960-8524 YR 2024 FD 2024 LK https://uvadoc.uva.es/handle/10324/73261 UL https://uvadoc.uva.es/handle/10324/73261 LA eng NO Bioresource Technology, septiembre 2024, vol. 408, 131180 NO Producción Científica DS UVaDOC RD 05-feb-2025