RT info:eu-repo/semantics/article
T1 Silica-Calcium-Alginate Hydrogels for the Co-Immobilization of Glucose Oxidase and Catalase to Reduce the Glucose in Grape Must
A1 del Bosque, David
A1 Vila Crespo, Josefina María
A1 Ruipérez Prádanos, Violeta
A1 Fernández Fernández, Encarnación
A1 Rodríguez Nogales, José Manuel
K1 encapsulation; siliceous material; hybrid capsule; sol-gel network; organic-inorganic gel
AB Higher temperatures due to climate change are causing greater sugar production in grapes and more alcoholic wines. The use of glucose oxidase (GOX) and catalase (CAT) in grape must is a biotechnological green strategy to produce reduced-alcohol wines. GOX and CAT were effectively co-immobilized by sol-gel entrapment in silica-calcium-alginate hydrogel capsules. The optimal coimmobilizationconditions were achieved at a concentration of the colloidal silica, sodium silicate andsodium alginate of 7.38%, 0.49% and 1.51%, respectively, at pH 6.57. The formation of a porous silicacalcium-alginate structure was confirmed by environmental scanning electron microscopy and the elemental analysis of the hydrogel by X-ray spectroscopy. The immobilized GOX showed a Michaelis–Menten kinetic, while the immobilized CAT fits better to an allosteric model. Immobilization also conferred superior GOX activity at low pH and temperature. The capsules showed a good operationalstability, as they could be reused for at least 8 cycles. A substantial reduction of 26.3 g/L of glucose was achieved with encapsulated enzymes, which corresponds to a decrease in potential alcoholic strength of must of about 1.5% vol. These results show that co-immobilized GOX and CAT in silica-calcium-alginate hydrogels is a promising strategy to produce reduced-alcohol wines.
PB MDPI
SN 2310-2861
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/73492
UL https://uvadoc.uva.es/handle/10324/73492
LA eng
NO Gels 2023, 9,320
NO Producción Científica
DS UVaDOC
RD 05-feb-2025