RT info:eu-repo/semantics/article
T1 Pharynx mitochondrial [Ca2+] dynamics in live C. elegans worms during aging
A1 Álvarez Illera, María Pilar
A1 García Casas, Paloma
A1 Arias del Val, Jessica
A1 Fonteriz García, Rosalba Inés
A1 Álvarez Martín, Javier
A1 Montero, Mayte
AB Progressive decline in mitochondrial function is generally considered one of the hallmarks of aging. We have expressed a Ca2+ sensor in the mitochondrial matrix of C. elegans pharynx cells and we have measured for the first time mitochondrial [Ca2+] ([Ca2+]M) dynamics in the pharynx of live C. elegans worms during aging. Our results show that worms stimulated with serotonin display a pharynx [Ca2+]M oscillatory kinetics that includes both high frequency oscillations (up to about 1Hz) and very prolonged "square-wave" [Ca2+]M increases, indicative of energy depletion of the pharynx cells. Mitochondrial [Ca2+] is therefore able to follow "beat-to-beat" the fast oscillations of cytosolic [Ca2+]. The fast [Ca2+]M oscillations kept steady frequency values during the whole worm life, from 2 to 12 days old, but the height and width of the peaks was progressively reduced. [Ca2+]M oscillations were also present with similar kinetics in respiratory chain complex I nuo-6 mutant worms, although with smaller height and frequency than in the controls, and larger width. In summary, Ca2+ fluxes in and out of the mitochondria are relatively well preserved during the C. elegans life, but there is a clear progressive decrease in their magnitude during aging. Moreover, mitochondrial Ca2+ fluxes were smaller in nuo-6 mutants with respect to the controls at every age and decreased similarly during aging.
YR 2017
FD 2017
LK https://uvadoc.uva.es/handle/10324/73911
UL https://uvadoc.uva.es/handle/10324/73911
LA eng
NO Alvarez-Illera P, García-Casas P, Arias-Del-Val J, Fonteriz RI, Alvarez J, Montero M. Pharynx mitochondrial [Ca2+] dynamics in live C. elegans worms during aging. Oncotarget. 2017 Jun 22;8(34):55889-55900. doi: 10.18632/oncotarget.18600. PMID: 28915560; PMCID: PMC5593531.
DS UVaDOC
RD 06-abr-2025