RT info:eu-repo/semantics/article
T1 Bacterial expression of biologically active recombinant musarmin 1 from bulbs of Muscari armeniacum L. and Miller
A1 Antolín, Pilar
A1 Girotti ., Alessandra
A1 Arias Vallejo, Francisco Javier
A1 Barriuso, Begoña
A1 Jiménez López, María del Pilar
A1 Rojo, María Ángeles
A1 Girbés Juan, Tomás
K1 Ribosome-inactivating protein; Musarmin; Expression; Purification; 28 S rRNA N-glycosidase
AB Musarmins are type 1 ribosome-inactivating proteins with N-glycosidase activity on the 28 S rRNA that are present in bulbs of Muscari armeniacum L. and Miller at rather low concentrations. In the present work, a cDNA fragment coding for musarmin 1 was sub-cloned and expressed in Escherichia coli. The recombinant protein (rMU1) was synthesised as a polypeptide of 295 amino acids that was delivered to the periplasm and processed. Recombinant musarmin 1 present in the periplam has two forms: insoluble with a molecular mass of 29,423 and soluble with a molecular mass of 29,117 because of a small proteolytic shortening with respect to the insoluble one, presumably in the C-terminal. The yield of protein homogeneous by polyacrylamide gel electrophoresis was 23 mg l−1 of bacterial culture. The recombinant musarmin 1 forms isolated from both the soluble and the insoluble (upon refolding) fractions retained full translational inhibitory and 28 S rRNA N-glycosidase activities as compared with the native protein. The recombinant protein displayed great stability towards trypsin, collagenase, rat plasma and rat liver protein extract, butwas sensitive to the action of papain and proteinase K. The easy availability and full activity of the recombinant musarmin 1 makes it a good candidate for the preparation of immunotoxins for targeted therapy and for the construction of transgenic plants expressing it as antipathogenic agent.
PB Elsevier
SN ISSN 0168-1656,
YR 2004
FD 2004
LK https://uvadoc.uva.es/handle/10324/74197
UL https://uvadoc.uva.es/handle/10324/74197
LA eng
NO Journal of Biotechnology 112, 313-322 (2004)
DS UVaDOC
RD 22-ene-2025