RT info:eu-repo/semantics/article
T1 Altered Arachidonate Distribution in Macrophages from Caveolin-1 Null Mice Leading to Reduced Eicosanoid Synthesis
A1 Astudillo, Alma M.
A1 Pérez Chacón, Gema
A1 Meana, Clara
A1 Balgoma Hernando, David
A1 Pol, Albert
A1 del Pozo, Miguel A.
A1 Balboa, María A.
A1 Balsinde, Jesús
AB In this work we have studied the effect of caveolin-1 deficiency on the mechanisms that regulate free arachidonic acid (AA) availability. The results presented here demonstrate that macrophages from caveolin-1-deficient mice exhibit elevated fatty acid incorporation and remodeling and a constitutivelyincreased CoA-independent transacylase activity. Mass spectrometry-based lipidomic analyses reveal stable alterations in the profile of AA distribution among phospholipids, manifested by reduced levels of AA in choline glycerophospholipids but elevated levels in ethanolamine glycerophospholipids and phosphatidylinositol. Furthermore, macrophages from caveolin-1 null mice show decreased AA mobilization and prostaglandin E2 and LTB4 production upon cell stimulation. Collectively, these results provide insight into the role of caveolin-1 in AA homeostasis and suggest an important role for this protein in the eicosanoid biosynthetic response.
SN 0021-9258
YR 2011
FD 2011
LK https://uvadoc.uva.es/handle/10324/74272
UL https://uvadoc.uva.es/handle/10324/74272
LA eng
NO Journal of Biological Chemistry, octubre 2011, vol. 286, n. 40, p. 35299-35307
DS UVaDOC
RD 29-ene-2025