RT info:eu-repo/semantics/article
T1 Genome-wide identification and characterization of Fusarium circinatum-responsive lncRNAs in Pinus radiata
A1 Zamora Ballesteros, Cristina
A1 Martín García, Jorge
A1 Suárez Vega, Aroa
A1 Díez Casero, Julio Javier
K1 Pinus radiata
K1 Fusarium circinatum
K1 lncRNA
K1 Transcriptomics network
K1 Conifer defence
K1 RNA-Seq
K1 3106.08 Silvicultura
K1 3108 Fitopatología
AB BackgroundOne of the most promising strategies of Pine Pitch Canker (PPC) management is the use of reproductive plant material resistant to the disease. Understanding the complexity of plant transcriptome that underlies the defence to the causal agent Fusarium circinatum, would greatly facilitate the development of an accurate breeding program. Long non-coding RNAs (lncRNAs) are emerging as important transcriptional regulators under biotic stresses in plants. However, to date, characterization of lncRNAs in conifer trees has not been reported. In this study, transcriptomic identification of lncRNAs was carried out using strand-specific paired-end RNA sequencing, from Pinus radiata samples inoculated with F. circinatum at an early stage of infection.ResultsOverall, 13,312 lncRNAs were predicted through a bioinformatics approach, including long intergenic non-coding RNAs (92.3%), antisense lncRNAs (3.3%) and intronic lncRNAs (2.9%). Compared with protein-coding RNAs, pine lncRNAs are shorter, have lower expression, lower GC content and harbour fewer and shorter exons. A total of 164 differentially expressed (DE) lncRNAs were identified in response to F. circinatum infection in the inoculated versus mock-inoculated P. radiata seedlings. The predicted cis-regulated target genes of these pathogen-responsive lncRNAs were related to defence mechanisms such as kinase activity, phytohormone regulation, and cell wall reinforcement. Co-expression network analysis of DE lncRNAs, DE protein-coding RNAs and lncRNA target genes also indicated a potential network regulating pectinesterase activity and cell wall remodelling.ConclusionsThis study presents the first comprehensive genome-wide analysis of P. radiata lncRNAs and provides the basis for future functional characterizations of lncRNAs in relation to pine defence responses against F. circinatum.
PB BMC
SN 1471-2164
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/74633
UL https://uvadoc.uva.es/handle/10324/74633
LA eng
NO BMC Genomics, March 2022, vol. 23, e194.
NO Producción Científica
DS UVaDOC
RD 09-mar-2025