RT info:eu-repo/semantics/article
T1 Real-time Loop-mediated Isothermal Amplification Assay for Rapid Detection of            Fusarium Circinatum
A1 Stehlíková, Dagmar
A1 Luchi, Nicola
A1 Aglietti, Chiara
A1 Pepori, Alessia Lucia
A1 Díez Casero, Julio Javier
A1 Santini, Alberto
K1 Elongation factor gene
K1 Field-deployable
K1 Invasive species
K1 Isothermal amplification
K1 LAMP
K1 Pine pitch canker
K1 3106 Ciencia Forestal
K1 3108 Fitopatología
AB Fusarium circinatum is the causal agent of pitch canker, a lethal disease of pine and other conifers. Since F. circinatum is a quarantine organism, its timely detection could efficiently prevent its introduction into new areas or facilitate spread management in already infected sites. In this study, we developed a sequence-specific probe loop-mediated isothermal amplification (LAMP) assay for F. circinatum using a field-deployable portable instrument. The assay was able to recognize the pathogen in host tissues in just 30 min, and the sensitivity of the assay made it possible to detect even small amounts of F. circinatum DNA (as low as 0.5 pg/μl). The high efficiency of this method suggests its use as a standard diagnostic tool during phytosanitary controls.
PB Taylor & Francis
SN 0736-6205
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/74636
UL https://uvadoc.uva.es/handle/10324/74636
LA eng
NO BioTechniques, 2020, vol. 69, n.1, p. 11-17.
NO Producción Científica
DS UVaDOC
RD 09-mar-2025