RT info:eu-repo/semantics/article
T1 A coating based on bioactive compounds from streptomyces spp. and chitosan oligomers to control botrytis cinerea preserves the quality and improves the shelf life of table grapes
A1 Buzon Duran, Laura
A1 Sánchez Hernández, Eva
A1 Sánchez Báscones, Mercedes
A1 García González, María Cruz
A1 Hernández Navarro, Salvador
A1 Correa Guimaraes, Adriana
A1 Martín Ramos, Pablo
AB Botrytis cinerea is the most harmful postharvest disease of table grapes. Among thestrategies that can be envisaged for its control, the use of coatings based on natural products isparticularly promising. The study presented herein focuses on the assessment of the antagonisticcapacity of two Streptomyces species and their culture filtrates against B. cinerea. Firstly, thesecondary metabolites were characterized by gas chromatography–mass spectrometry, with N1-(4-hydroxybutyl)-N3-methylguanidine acetate and 2R,3S-9-[1,3,4-trihydroxy-2-butoxymethyl]guanineacetate as the main compounds produced by S. lavendofoliae DSM 40217; and cyclo(leucyloprolyl)and cyclo(phenylalanylprolyl) as the most abundant chemical species for S. rochei DSM 41729. Subsequently,the capacity of S. lavendofoliae DSM 40217 and S. rochei DSM 41729 to inhibit the growth ofthe pathogen was tested in dual culture plate assays, finding 85–90% inhibition. In agar dilution tests,their culture filtrates resulted in effective concentration values (EC90) in the 246–3013  g mL�����1 range.Upon the formation of conjugate complexes with chitosan oligomers (COS) to improve solubilityand bioavailability, a synergistic behavior was observed, resulting in lower EC90 values, rangingfrom 201 to 953 microgramos/mL. Ex situ tests carried out on ‘Timpson’ and ‘Red Globe’ table grapes using the conjugate complexes as coatings were found to maintain the turgor of the grapes and delaythe appearance of the pathogen by 10-15 days at concentrations in the 750-1000 microgramos/mL range.Hence, the conjugate complexes of COS and the selected Streptomyces spp. culture filtrates may beput forward as promising protection treatments for the sustainable control of gray mold.
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/74696
UL https://uvadoc.uva.es/handle/10324/74696
LA eng
NO Plants, enero 2023, vol. 12, 577
DS UVaDOC
RD 09-mar-2025