RT info:eu-repo/semantics/article
T1 wnt5a is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis
A1 Kaiser, Karol
A1 Gyllborg, Daniel
A1 Procházka, Jan
A1 Salašová, Alena
A1 Kompaníková, Petra
A1 Molina, Francisco Lamus
A1 Laguna-Goya, Rocio
A1 Radaszkiewicz, Tomasz
A1 Harnoš, Jakub
A1 Procházková, Michaela
A1 Potěšil, David
A1 Barker, Roger A.
A1 Gato Casado, Ángel Luis
A1 Zdráhal, Zbyněk
A1 Sedláček, Radislav
A1 Arenas, Ernest
A1 Villaescusa, J. Carlos
A1 Bryja, Vítězslav
K1 Embriología Experimental
K1 WNT5A signaling
K1 Cerebrospinal fluid (CSF)
K1 Lipoprotein transport
K1 Hindbrain morphogenesis
K1 Neurodevelopment
K1 2401.07 Embriología Animal
AB WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) of the developing hindbrain, but not the telencephalon, in both mouse and human. Since the ChP produces and secretes the cerebrospinal fluid (CSF), we examine the presence of WNT5A in the CSF and find that it is associated with lipoprotein particles rather than exosomes. Moreover, since the CSF flows along the apical surface of hindbrain progenitors not expressing Wnt5a, we examined whether deletion of Wnt5a in the ChP controls their function and find that cerebellar morphogenesis is impaired. Our study thus identifies the CSF as a route and lipoprotein particles as a vehicle for long-range transport of biologically active WNT in the central nervous system.
PB Nature Publishing Group
SN 2041-1723
YR 2019
FD 2019
LK https://uvadoc.uva.es/handle/10324/74708
UL https://uvadoc.uva.es/handle/10324/74708
LA spa
NO WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis. Nat Commun. 2019 Apr 2;10(1):1498
NO Producción Científica
DS UVaDOC
RD 05-abr-2025