RT info:eu-repo/semantics/article
T1 “In-situ” formation of elastin-like recombinamer hydrogels with tunable viscoelasticity through efficient one-pot process
A1 Hamed Misbah, Mohamed
A1 Quintanilla Sierra, Luis
A1 Alonso Rodrigo, Matilde
A1 Rodríguez Cabello, José Carlos
A1 Santos García, María Mercedes
K1 ELRs
K1 Hydrogel
K1 Amidation reaction
K1 Viscoelastic
K1 23 Química
K1 32 Ciencias Médicas
AB Despite the remarkable progress in the generation of recombinant elastin-like (ELR) hydrogels, further improvements are still required to enhance and control their viscoelasticity, as well as limit the use of expensive chemical reagents, time-consuming processes and several purification steps. To alleviate this issue, the reactivity of carboxylic groups from glutamic (E) acid distributed along the hydrophilic block of an amphiphilic ELR (coded as E50I60) with amine groups has been studied through a one-pot amidation reaction in aqueous solutions, for the first time. By means of this approach, immediate conjugation of E50I60 with molecules containing amine groups has been performed with a high yield, as demonstrated by the 1H NMR and MALDI-TOF spectroscopies. This has resulted in the preparation of viscoelastic irreversible hydrogels through the “in-situ” cross-linking of E50I60 with another ELR (coded as VKV24) containing amine groups from lysines (K). The rheology analysis demonstrated that the gelation process takes place following a dual mechanism dependent on the ELR concentration: physical cross-linking of I60 block through the hydrophobic interactions, and covalent cross-linking of E50I60 with VKV24 through the amidation reaction. While the chemical network formed between the hydrophilic E50 block and VKV24 ELR preserves the elasticity of ELR hydrogels, the self-assembly of the I60 block through the hydrophobic interactions provides a tunable physical network. The presented investigation serves as a basis for generating ELR hydrogels with tunable viscoelastic properties promising for tissue regeneration, through an ‘‘in-situ”, rapid, scalable, economically and feasible one-pot method.
PB Elsevier
SN 2590-0064
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/74822
UL https://uvadoc.uva.es/handle/10324/74822
LA eng
NO Materials Today Bio, April 2024, vol. 25, 100999
NO Producción Científica
DS UVaDOC
RD 23-feb-2025