RT info:eu-repo/semantics/article
T1 Genome-wide identification and in-silico expression analysis of CCO gene family in sunflower (Helianthus annuus) against abiotic stress
A1 Sami, Adnan
A1 Zeeshan Haider, Muhammad
A1 Shafiq, Muhammad
A1 Sadiq, Saleh
A1 Ahmad, Farooq
K1 Carotenoid cleavage oxygenases
K1 RNA seq analysis
K1 Transcription factor
K1 Helianthus annuus
K1 9-cis- epoxycarotenoid dioxygenases
K1 Carotenoid cleavage dioxygenases
K1 31 Ciencias Agrarias
AB Carotenoid cleavage oxygenases (CCOs) enzymes play an important role in plant growth and development by produc-ing a wide array of apocarotenoids and their derivatives. These compounds are vital for colouring flowers and fruits andsynthesizing plant hormones such as abscisic acid and strigolactones. Despite their importance, the gene family respon-sible for CCO enzymes in sunflowers has not been identified. In this study, we identify the CCO genes of the sunflowerplant to fill this knowledge gap. Phylogenetic and synteny analysis indicated that the Helianthus annuus CCO (HaCCO)genes were conserved in different plant species and they could be divided into three subgroups based on their conserveddomains. Analysis using MEME tool and multiple sequence alignment identified conserved motifs in the HaCCO genesequence. Cis-regulatory elements (CREs) analysis of the HaCCO genes indicated the presence of various responsiveelements related to plant hormones, development, and responses to both biotic and abiotic stresses. This implies thatthese genes may respond to plant hormones, developmental cues, and drought stress, offering potential applications in thedevelopment of more resistant crops. Genes belonging to the 9-cis-epoxy carotenoid dioxygenases (NCED) subgroupspredominantly exhibited chloroplast localization, whereas the genes found in other groups are primarily localized in thecytoplasm. These 21 identified HaCCOs were regulated by 60 miRNAs, indicating the crucial role of microRNAs in generegulation in sunflowers. Gene expression analysis under drought stress revealed significant up-regulation of HaNCED16and HaNCED19, genes that are pivotal in ABA hormone biosynthesis. During organ-specific gene expression analysis,HaCCD12 and HaCCD20 genes exhibit higher activity in leaves, indicating a potential role in leaf pigmentation. Thisstudy provides a foundation for future research on the regulation and functions of the CCO gene family in sunflower andbeyond. There is potential for developing molecular markers that could be employed in breeding programs to create newsunflower lines resistant to biotic and abiotic stresses.
PB Springer
SN 0167-4412
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/75133
UL https://uvadoc.uva.es/handle/10324/75133
LA eng
NO Plant Molecular Biology, 2024, vol. 114, n. 2
NO Producción Científica
DS UVaDOC
RD 15-may-2025