RT info:eu-repo/semantics/article
T1 Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
A1 Herrero Lobo, Raquel
A1 Fernández González, Nuria
A1 Marcos Rodrigo, Eva
A1 Martínez, María Alejandra
A1 García Encina, Pedro Antonio
A1 Rodero Raya, María del Rosario
A1 Muñoz Torre, Raúl
A1 Bordel Velasco, Sergio
K1 ectoine
K1 hydroxyectoine
K1 methanotrophic bacteria
K1 Taylor flow
K1 methane
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB BACKGROUND: The production of compatible solutes, such as ectoine and hydroxyectoine, is of great interest due to their industrial and biotechnological applications. Methylomicrobium alcaliphilum was genetically engineered to replace a native gene with a heterologous one, aiming to enhance ectoine production. This study focuses on the optimization of bioreactor conditions to maximize the microbial production of these metabolites from methane.RESULTS: The engineered strain (M. alcaliphilum PstEctD) was cultured in a Taylor flow bioreactor under varying gas recirculation flow rates. Increased flow rates enhanced methane consumption, biomass concentration, and ectoine production. The highest production of ectoine (32 mg/g-VSS) and hydroxyectoine (272 mg/g-VSS) was observed at a flow rate of 0.7 L min−1, while methane removal efficiency improved from 30% to over 60% as flow rates increased.CONCLUSIONS: Optimizing bioreactor conditions, particularly gas recirculation flow rates, significantly improved both the efficiency of methane consumption and the production of ectoine derivatives. This work provides a scalable approach for the sustainable production of compatible solutes from methane, offering potential applications in biotechnological processes utilizing renewable carbon sources. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
PB Wiley
SN 0268-2575
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/75154
UL https://uvadoc.uva.es/handle/10324/75154
LA eng
NO Journal of Chemical Technology & Biotechnology, [Early View]
NO Producción Científica
DS UVaDOC
RD 01-mar-2025