RT info:eu-repo/semantics/article
T1 Biological variability of human intraepithelial lymphocytes throughout the human gastrointestinal tract in health and coeliac disease
A1 Fiz López, Aida
A1 Prado, Ángel de
A1 Arribas Rodríguez, Elisa
A1 García Alonso, Francisco Javier
A1 Izquierdo, Sandra
A1 Martín Muñoz, Álvaro
A1 Garrote Adrados, José Antonio
A1 Arranz Sanz, Eduardo
A1 Barrio, Jesús
A1 Fernández Salazar, Luis Ignacio
A1 Bernardo Ordiz, David
K1 coeliac disease
K1 IL- 15Rα
K1 intraepithelial lymphocytes
K1 2407 Biología Celular
K1 2412 Inmunología
K1 3205.03 Gastroenterología
AB Background: Intraepithelial lymphocytes are the first line of defence of the human intestinal immune system. Besides, their composition is altered on patients with coeliac disease (CD), so they are considered as biomarkers with utility on their diagnose and/or monitoring. Our aim is to address their variability through the human gastrointestinal tract in health and characterized them in further depth in the coeliac duodenum.Methods: Intraepithelial lymphocytes were isolated from human gastric, duodenal, ileal and colonic biopsies, then stained with specific antibodies and acquired by flow cytometry.Results: Our results confirmed that the profile of Intraepithelial lymphocytes change through the length of the human gastrointestinal tract. Besides and given the central role that Interleukin-15 (IL-15) elicits on CD pathogenesis; we also assessed the expression of its receptor revealing that there was virtually no functional IL-15 receptor on duodenal Intraepithelial lymphocytes. Nevertheless and contrary to our expectations, the active IL-15 receptor was not increased either on Intraepithelial lymphocytes from CD patients.Conclusions: IL-15 might require additional stimulus to activate intraepithelial lymphocytes. These findings may provide novel tools to aid on a CD diagnosis and/or monitoring, at the time that provide the bases to perform functional studies in order of getting a deeper insight in the specific function that Intraepithelial lymphocytes elicit on CD pathogenesis.
PB Wiley
SN 0014-2972
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/75159
UL https://uvadoc.uva.es/handle/10324/75159
LA eng
NO European Journal of Clinical Investigation, 2024, vol. 54, n. 12, e14304
NO Producción Científica
DS UVaDOC
RD 03-mar-2025