RT info:eu-repo/semantics/article
T1 Evaluation of the extract of pseudopithomyces chartarum to be used as biocontrol agent against phytophthora cinnamomi in Lupinus luteus
A1 García Latorre, Carlos
A1 Rodrigo, Sara
A1 SantaMaría Becerril, Óscar
K1 Fungal endophytes
K1 Secondary metabolites
K1 Plant protection
K1 Pseudopithomyces chartarum
K1 Lupinus luteus
K1 Phytophthora cinnamomi
K1 31 Ciencias Agrarias
AB Endophytic fungi offer promising opportunities for a sustainable disease management in agricultural systems, often relatedto their secondary metabolites. In this study, the potential of the metabolites produced in vitro by Pseudopithomyceschartarum to protect the forage crop Lupinus luteus against the soil-borne pathogen Phytophthora cinnamomi was evalu-ated in different in vitro and in planta tests. The fungal extracts containing the metabolites were applied by two differ-ent procedures: to seeds by immersion (mycopriming) and to seedlings by spraying (post-emergence). The antagonismbetween the endophyte fitrates/extracts and the pathogen was studied by dual culture assays and the minimal inhibitoryconcentration. Other filtrate/extract traits, such as its phytohormones production and nutrient mobilization, were also ana-lyzed. The metabolites contained in the extract were tentatively identified by mass spectrometry. The endophyte’s filtratesand extracts exhibited significant inhibition on the P. cinnamomi growth in vitro. Additionally, seed mycopriming andpost-emergence extract application positively influenced seed germination, reduced disease severity, and enhanced severalgrowth parameters in treated seedlings. Auxins and gibberellins production, and phosphate solubilization activity wereobserved in the endophyte, traits potentially contributing to its biocontrol efficacy. The bioactive compounds ciclo(L-Phe-L-Pro), dihydroabikoviromycin, medelamine A, herniarin, and Piptamine, all with antimicrobial properties, were tenta-tively identified in the extracts, but further research is needed to confirm this identity and to evaluate the extract efficacyunder field conditions. This study highlights the potential of certain endophytic fungi as sustainable and environmentallyfriendly alternatives for a disease management in agriculture
PB Springer
SN 0718-9508
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/75223
UL https://uvadoc.uva.es/handle/10324/75223
LA eng
NO Journal of Soil Science and Plant Nutrition, 2024, vol. 24, n. 4, p. 6325-6337
NO Producción Científica
DS UVaDOC
RD 10-abr-2025