RT info:eu-repo/semantics/doctoralThesis
T1 Efecto del flúor en la adhesión bacteriana a materiales de restauración a base de ionómero de vidrio de nueva generación y materiales de cementación a base de resina
A1 Marcano Navascués, Rosalía
A2 Universidad de Valladolid. Escuela de Doctorado
K1 Odontología
K1 Oral diseases
K1 Enfermedades orales
K1 Bacterial adhesion
K1 Adhesión bacteriana
K1 Fluoride agents
K1 Agentes fluorados
K1 Biofilms
K1 Biofilms
K1 32 Ciencias Médicas
AB ABSTRACT: Oral diseases, which affect billions of people worldwide, are largely preventable but remain prevalent due to factors such as a diet high in sugars and poor oral hygiene, which can promote dysbiosis. Dental restorations, while effective in rehabilitating function and aesthetics, are susceptible to bacterial adhesion, increasing the risk of oral and eventually systemic diseases. Reducing this adhesion through the use of fluoride agents could improve the durability of restorations and overall oral health.AIM: The primary aim of the research is to evaluate whether the application of fluoride agents to dental restoration materials can significantly reduce bacterial adhesion and proliferation, thereby contributing to greater durability of restorations and a reduction in the recurrence of oral diseases.METHODS: The study employed an in vitro model to assess bacterial adhesion to the surfaces of dental materials treated with fluoride agents (NaF-CPP-ACP). Three common bacterial strains involved in the development of dental caries and periodontal disease were selected: Streptococcus mutans, Actinomyces viscosus, and Lactobacillus acidophilus. The bacteria were cultured in specific media, and samples of restorative material (hybrid glass) and cementation material (resin) treated with fluoride agents were prepared. Bacterial adhesion was evaluated through optical density measurement, observation with Fluorescence Microscopy (FM), and colony-forming unit (CFU) counts.RESULTS:The results showed that both materials allowed moderate bacterial growth, which was lower in the resin-based cement. The application of a fluoride gel to the hybrid glass restorative material effectively reduced the adhesion of S. mutans compared to other treatments or untreated samples. However, this strategy was not as effective for A. viscosus and L. acidophilus, where slight growth was observed. The fluoride varnish, on the other hand, performed better on the resin-based cementation material, showing higher bacterial adhesion overall, especially for A. viscosus and L. acidophilus. In polymicrobial cultures, the samples treated with the gel exhibited low adhesion levels, suggesting that this treatment could be more effective in preventing biofilm formation in the presence of multiple microorganisms.CONCLUSION: The study concludes that both materials treated with fluoride agents showed some reduction in bacterial adhesion, with efficacy varying depending on the microorganism type, the restorative material, and the treatment applied. These findings suggest that the application of NaF-CPP-ACP-based fluoride agents could be a valuable strategy in clinical practice to improve the durability of dental restorations and moderate biofilm formation.
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/75322
UL https://uvadoc.uva.es/handle/10324/75322
LA spa
NO Escuela de Doctorado
DS UVaDOC
RD 02-abr-2025