RT info:eu-repo/semantics/article
T1 Blood-brain barrier leakage in renovascular hypertensive rats: a quantitative MRI analysis
A1 Alonso-Alonso, María Luz
A1 Sampedro-Viana, Ana
A1 Fernandez-Bueno, Ivan
A1 Pérez-Mato, María
A1 Correa-Paz, Clara
A1 Pérez-Gayol, Lara
A1 Romaus-Sanjurjo, Daniel
A1 Ouro, Alberto
A1 Blanco-Santero, Javier
A1 Oblitas, Crhistian-Mario
A1 Castillo, José
A1 Hervella, Pablo
A1 Iglesias-Rey, Ramón
AB Hypertension is a modifiable risk factor for cerebral small vessel disease (cSVD) whichleads to blood-brain barrier (BBB) dysfunction. This study used gadolinium-enhancedMRI T1 and T2 in stroke-prone renovascular hypertensive rats (RHRSP) to quantify BBBleakage and brain lesions. Serum inflammation and endothelial disruption biomarkerswere assessed. Brain aquaporin 4 (AQ4) and retinal morphology were evaluated byhistology. RHRSP showed higher systolic blood pressure from the first post-surgical week(134.1 ± 16.6 vs 113.5 ± 11.4 mmHg; p=0.041). Gadolinium extravasation was increasedin the whole brain of RHRSP (p<0.05), observing cerebral lesions in 50% of them.Regarding biomarkers, TNF-α was increased (13.5 ± 2.2 vs 11.1 ± 3.5 pg/ml, p=0.031)while soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) was lower(14.5 ± 2.2 vs 18.9 ± 2.5 pg/ml, p<0.001) in the acute hypertension phase. AQ4 wasincreased in the RHRSP brain (1.6 ± 0.7 vs 1.0 ± 0.2 NFU; p=0.001). Both retinal nuclearlayers were reduced in RHRSP (p<0.05). Gadolinium-enhanced MRI allows the detectionof BBB leakage during the establishment of RHRSP. BBB dysfunction is consistent withincreased AQ4 and decreased sTWEAK release. Therefore, this animal model is usefulfor testing potential treatments at cSVD initial stages.
SN 1053-8119
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/75783
UL https://uvadoc.uva.es/handle/10324/75783
LA eng
NO Marıa Luz Alonso-Alonso, Ana Sampedro-Viana, Ivan Fernandez-Bueno, Marıa Perez-Mato, Clara Correa-Paz, Lara Perez-Gayol, Daniel Romaus-Sanjurjo, Alberto Ouro, Javier Blanco-Santero, Crhistian-Mario Oblitas, Jose Castillo, Pablo Hervella, Ramon Iglesias-Rey. Blood-brain barrier leakage in renovascular hypertensive rats: a quantitative MRI analysis, NeuroImage (2025), doi: https://doi.org/10.1016/j.neuroimage.2025.121283
NO Producción Científica
DS UVaDOC
RD 25-may-2025