RT info:eu-repo/semantics/doctoralThesis T1 Identificación de nuevas estrategias terapéuticas en la neuroinflamación A1 Simón De la Fuente, María Inmaculada A2 Universidad de Valladolid. Escuela de Doctorado K1 Esclerosis múltiple K1 Sclerosis Multiple K1 Esclerosis Múltiple K1 EAE K1 EAE K1 Neuroinflammation K1 Neuroinflamación K1 Microglia K1 Microglia K1 2302.21 Biología Molecular AB Multiple Sclerosis is an autoimmune demyelinating disease of the central nervous system (CNS). Currently, therapeutic strategies are aimed at reducing the disease and treating the acute flare, but in most cases they cause a large number of side effects without efficacy. The aim of this thesis has been to investigate the therapeutic effect of the natural compound MCP (modified citrus pectin) in the experimental model of MS, Experimental Autoimmune Encephalitis; The results of these studies show that the administration of MCP in mice protects against the development of EAE, reducing cellular and humoral immune responses and delaying the appearance of symptoms derived from the disease. The use of MCP reduced the formation of reactive oxygen species (ROS), inhibited the infiltration of immune cells and the secretion of proinflammatory mediators in CNS tissues. On the other hand, in the gastrointestinal system, this compound reduced the presence of proteins indicating a permeable intestine such as iFABP and sCD14 in serum and increased the expression levels of the anti-inflammatory cytokine IL-25, improving the intestinal barrier damaged by EAE.Another objective of this thesis has been to analyze the therapeutic application of NaE (phenolic extract of yellow laurel) in a microglial cell model, which represents the cells present in the CNS. The results showed that the use of NaE reduced the presence of induced proinflammatory mediators such as TNF α, IL-1β, IL-6, COX-2 and INOS, as well as the accumulation of NO, while promoting the secretion of IL-10 and the expression of YM-1. NaE also reduced the accumulation of ROS, prevented the phosphorylation of NF-κB and MAPK and decreased cell migration, without affecting the phagocytic capacity of the cells.These results together demonstrate the therapeutic potential of these two natural compounds, MCP and NaE, to be used in autoimmune and inflammatory diseases, due to their antioxidant and immunomodulatory effects. YR 2025 FD 2025 LK https://uvadoc.uva.es/handle/10324/75874 UL https://uvadoc.uva.es/handle/10324/75874 LA spa NO Escuela de Doctorado DS UVaDOC RD 13-jun-2025