RT info:eu-repo/semantics/doctoralThesis
T1 Identificación de nuevas estrategias terapéuticas en la neuroinflamación
A1 Simón De la Fuente, María Inmaculada
A2 Universidad de Valladolid. Escuela de Doctorado
K1 Esclerosis múltiple
K1 Sclerosis Multiple
K1 Esclerosis Múltiple
K1 EAE
K1 EAE
K1 Neuroinflammation
K1 Neuroinflamación
K1 Microglia
K1 Microglia
K1 2302.21 Biología Molecular
AB Multiple Sclerosis is an autoimmune demyelinating disease of the central nervous system (CNS). Currently, therapeutic strategies are aimed at reducing the disease and treating the acute flare, but in most cases they cause a large number of side effects without efficacy. The aim of this thesis has been to investigate the therapeutic effect of the natural compound MCP (modified citrus pectin) in the experimental model of MS, Experimental Autoimmune Encephalitis; The results of these studies show that the administration of MCP in mice protects against the development of EAE, reducing cellular and humoral immune responses and delaying the appearance of symptoms derived from the disease. The use of MCP reduced the formation of reactive oxygen species (ROS), inhibited the infiltration of immune cells and the secretion of proinflammatory mediators in CNS tissues. On the other hand, in the gastrointestinal system, this compound reduced the presence of proteins indicating a permeable intestine such as iFABP and sCD14 in serum and increased the expression levels of the anti-inflammatory cytokine IL-25, improving the intestinal barrier damaged by EAE.Another objective of this thesis has been to analyze the therapeutic application of NaE (phenolic extract of yellow laurel) in a microglial cell model, which represents the cells present in the CNS. The results showed that the use of NaE reduced the presence of induced proinflammatory mediators such as TNF α, IL-1β, IL-6, COX-2 and INOS, as well as the accumulation of NO, while promoting the secretion of IL-10 and the expression of YM-1. NaE also reduced the accumulation of ROS, prevented the phosphorylation of NF-κB and MAPK and decreased cell migration, without affecting the phagocytic capacity of the cells.These results together demonstrate the therapeutic potential of these two natural compounds, MCP and NaE, to be used in autoimmune and inflammatory diseases, due to their antioxidant and immunomodulatory effects.
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/75874
UL https://uvadoc.uva.es/handle/10324/75874
LA spa
NO Escuela de Doctorado
DS UVaDOC
RD 14-jun-2025