RT info:eu-repo/semantics/article
T1 Continuous Valorization of Carbon Dioxide into the Fine Chemical Ectoine by Hydrogenovibrio marinus: A New Strategy for Pharmaceutical Production
A1 Huang Lin, Elisa
A1 Lebrero Fernández, Raquel
A1 Cantera Ruiz De Pellon, Sara
K1 Biotechnology
K1 Biomass
K1 Energy
K1 Assays
K1 Bacteria
K1 Carbon dioxide
K1 CO2 valorization
K1 Extremolytes
K1 Halophiles
K1 Hydrogen-Oxidizing Bacteria
K1 Knallgas Bacteria
AB Current challenges in biopharmaceutical manufacturing, such as ectoine production, include high operational costs and limited availability. Transitioning to processes that valorize renewable carbon sources like CO2 into ectoine can make production more sustainable and accessible to the economy and society. However, cell platforms that produce ectoine with CO2 still require bioprocess optimization and resilient microorganisms able to continuously maintain high ectoine yields and CO2 removals. A comprehensive screening of cultivation and operational strategies was conducted in six stirred-tank gas bioreactors using the strain Hydrogenovibrio marinus, a halophilic, fast-growing, hydrogenotrophic bacterium with low nutrient requirements. Gas residence times of 120 min at gas ratios of 10:40:50 CO2:H2:air (% v/v) and dilution rates of 0.25 d-1 boosted ectoine production and biomass growth during long-term operation. Under these conditions, ectoine productivity reached 5.0 ± 0.3 g m-3 d-1, with maximum specific ectoine contents of 134.0 ± 6.3 mgEct gbiomass-1, achieving yields similar to heterotrophic strains. This study demonstrates for the first time the feasibility of integrating ectoine production with continuous CO2 abatement using H2 as a clean and hazard-free energy source, which marks a significant advancement in sustainable ectoine manufacturing and CO2 circularity.
PB American Chemical Society
SN 0013-936X
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/76096
UL https://uvadoc.uva.es/handle/10324/76096
LA spa
NO Environmental Science and Technology, 2025, vol. 59, n. 10 p. 5088-5097
NO Producción Científica
DS UVaDOC
RD 25-jun-2025