RT info:eu-repo/semantics/article
T1 Spray‐induced gene silencing to control plant pathogenic fungi: A step‐by‐step guide
A1 Mosquera, Sandra
A1 Ginésy, Mireille
A1 Amin, Huma
A1 Bocos Asenjo, Irene Teresa
A1 Niño Sánchez, Jonatan
A1 Diez Hermano, Sergio
A1 Díez Casero, Julio Javier
K1 Fungal plant pathogens
K1 Silenciamiento genético inducido por pulverización
K1 Spray‐ induced gene silencing (SIGS)
AB RNA interference (RNAi)-based control technologies are gaining popularity as potential alternatives to synthetic fungicides in the ongoing effort to manage plant pathogenic fungi. Among these methods, spray-induced gene silencing (SIGS) emerges as particularly promising due to its convenience and feasibility for development. This approach is a new technology for plant disease management, in which double-stranded RNAs (dsRNAs) targeting essential or virulence genes are applied to plants or plant products and subsequently absorbed by plant pathogens, triggering a gene silencing effect and the inhibition of the infection process. Spray-induced gene silencing has demonstrated efficacy in laboratory settings against various fungal pathogens. However, as research progressed from the laboratory to the greenhouse and field environments, novel challenges arose, such as ensuring the stability of dsRNAs and their effective delivery to fungal targets. Here, we provide a practical guide to SIGS for the control of plant pathogenic fungi. This guide outlines the essential steps and considerations needed for designing and assessing dsRNA molecules. It also addresses key challenges inherent to SIGS, including delivery and stability of dsRNA molecules, and how nanoencapsulation of dsRNAs can aid in overcoming these obstacles. Additionally, the guide underscores existing knowledge gaps that warrant further research and aims to provide assistance to researchers, especially those new to the field, encouraging the advancement of SIGS for the control of a broad range of fungal pathogens.
PB John Wiley & Sons Ltd.
SN 1672-9072
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/76174
UL https://uvadoc.uva.es/handle/10324/76174
LA eng
NO Journal of Integrative Plant Biology, 2025, vol. 67, n. 3. p. 801-825
NO Producción Científica
DS UVaDOC
RD 19-jul-2025