RT info:eu-repo/semantics/article
T1 Examining mixotrophic fermentation in fed‐batch mode for C1‐gas valorization
A1 Fernández Delgado, Marina
A1 Plaza Lazaro, Pedro Enrique
A1 García Cubero, María Teresa
A1 Lucas Yagüe, Susana
A1 Coca Sanz, Mónica
A1 López Linares, Juan Carlos
K1 Mixotrophic fermentation
K1 C1-gases
K1 Biotecnología
K1 Autotrophic fermentation
K1 C1-gases
K1 Clostridia spp
K1 fructose
K1 Mixotrophic fermentation
AB BACKGROUND: C1-gases like CO and CO2, significant contributors to climate change, offer the potential for sustainable bioconversion into valuable products. The study explored mixotrophic fermentation using C1-gases in fed-batch mode to improve the production of target compounds, focusing on Clostridium aceticum and Clostridium carboxidivorans. It aimed to overcome the limitations of conventional gas fermentation (autotrophic fermentation and without fed-batch mode) and assess the potential of mixotrophic substrates for enhancing yields. RESULTS: Results showed that mixotrophic fermentation with fructose as a co-substrate led to higher microbial growth in C. aceticum, increasing acetic acid (1200 versus 600 mg L−1) and ethanol (600 versus 0 mg L−1) production, compared to autotrophic fermentation. For C. carboxidivorans, constant CO consumption occurred in autotrophic and mixotrophic fermentation. Mixotrophic fermentation with fructose and C1-gases by C. carboxidivorans significantly boosted microbial growth and metabolic activity, increasing butanol (1600 versus 0 mg L−1) and butyric acid (2400 versus 1800 mg L−1) production, compared to autotrophic fermentation. CONCLUSIONS: The study highlights mixotrophic fermentation's potential to enhance C1-gas valorization. It provides insights into microbial behavior under varied substrate conditions, contributing to sustainable biomanufacturing practices for biofuel and high-value bioproducts. © 2025 Society of Chemical Industry (SCI).
PB John Wiley & Sons
SN 0268-2575
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/76272
UL https://uvadoc.uva.es/handle/10324/76272
LA eng
NO Journal of Chemical Technology and Biotechnology, 2025, vol. 100, n. 7. p. 1453-1462
NO Producción Científica
DS UVaDOC
RD 13-jul-2025